Strep Tag II Antikörper (FITC)

Produktnummer ABIN962832

Produktdetails

Target: Strep Tag II

Reaktivität: Bitte anfragen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: FITC

Applikation: Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Marke: THE™

Sequenz: NWSHPQFEK

Spezifität: THE^TM NWSHPQFEK Tag Antibody [FITC], mAb, Mouse recognizes N-terminal and C-terminal Strep II tagged fusion proteins.

Produktmerkmale: The THE^TM NWSHPQFEK Tag Antibody [FITC], mAb, Mouse is THE^TM NWSHPQFEK Tag Antibody conjugated with FITC. The conjugated antibody is suitable for detecting the expression level of NWSHPQFEK (Strep II tag) tagged fusion proteins.

Aufreinigung: Protein A affinity column

Immunogen: NWSHPQFEK peptide conjugated - KLH

Klon: 5A9F9

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
FACS: 1-3 µg for 1 x 10^6 cells
ICC/IF: 1-3 µg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/mL.

Buffer: PBS, pH 7.4, containing 10 mg/mL BSA and 0.02 % Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Avoid repeated freezing and thawing cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.

Antigendetails

Target: Strep Tag II

Substanzklasse: Tag

Hintergrund: Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. Strep II tag (NWSHPQFEK) is a nine amino acid peptide with high specificity and affinity towards streptavidin.