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Strep Tag II Antikörper (HRP)

WB, ELISA Wirt: Maus Monoclonal 5A9F9 HRP
Produktnummer ABIN962835
  • Target
    Strep Tag II
    Reaktivität
    Bitte anfragen
    Wirt
    • 8
    • 4
    Maus
    Klonalität
    • 8
    • 4
    Monoklonal
    Konjugat
    • 7
    • 2
    • 2
    • 1
    HRP
    Applikation
    • 11
    • 7
    • 3
    • 2
    • 2
    • 1
    Western Blotting (WB), ELISA
    Marke
    THE™
    Sequenz
    NWSHPQFEK
    Spezifität
    THETM NWSHPQFEK Tag Antibody [HRP], mAb, Mouse recognizes N-terminal and C-terminal Strep II tagged fusion proteins.
    Produktmerkmale
    The THETM NWSHPQFEK Tag Antibody [HRP], mAb, Mouse is THETM NWSHPQFEK Tag Antibody conjugated with Horseradish peroxidase (HRP). The conjugated antibody is suitable for detecting the expression level of NWSHPQFEK Tagged fusion proteins.
    Aufreinigung
    Protein A affinity column
    Immunogen
    NWSHPQFEK peptide conjugated - KLH
    Klon
    5A9F9
    Isotyp
    IgG1
  • Applikationshinweise
    Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
    Western blot: 0.2-1 µg/mL ELISA: 0.001-0.002 µg/mL
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/mL.
    Buffer
    PBS, pH 7.4, containing 10 mg/mL BSA and 0.01 % Thimerosal.
    Konservierungsmittel
    Thimerosal (Merthiolate)
    Vorsichtsmaßnahmen
    This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Avoid repeated freezing and thawing cycles.
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.
  • Target
    Strep Tag II
    Substanzklasse
    Tag
    Hintergrund
    Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. NWSHPQFEK (Strep II tag) is a nine amino acid peptide with high specificity and affinity towards streptavidin.
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