Strep Tag II Antikörper (HRP)

Produktnummer ABIN962835

Produktdetails

Target: Strep Tag II

Reaktivität: Bitte anfragen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: HRP

Applikation: Western Blotting (WB), ELISA

Marke: THE™

Sequenz: NWSHPQFEK

Spezifität: THE^TM NWSHPQFEK Tag Antibody [HRP], mAb, Mouse recognizes N-terminal and C-terminal Strep II tagged fusion proteins.

Produktmerkmale: The THE^TM NWSHPQFEK Tag Antibody [HRP], mAb, Mouse is THE^TM NWSHPQFEK Tag Antibody conjugated with Horseradish peroxidase (HRP). The conjugated antibody is suitable for detecting the expression level of NWSHPQFEK Tagged fusion proteins.

Aufreinigung: Protein A affinity column

Immunogen: NWSHPQFEK peptide conjugated - KLH

Klon: 5A9F9

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.2-1 µg/mL ELISA: 0.001-0.002 µg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/mL.

Buffer: PBS, pH 7.4, containing 10 mg/mL BSA and 0.01 % Thimerosal.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.

Antigendetails

Target: Strep Tag II

Substanzklasse: Tag

Hintergrund: Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. NWSHPQFEK (Strep II tag) is a nine amino acid peptide with high specificity and affinity towards streptavidin.