His Tag Antikörper

Produktnummer ABIN964552

Hersteller Produkt- Nr.: 200-301-382

Produktdetails anti-His Tag Antikörper

Target: His Tag

Reaktivität: Bitte anfragen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser His Tag Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Hersteller Produkt- Nr.: 200-301-382

Hersteller: Rockland

Verwendungszweck: 6X His Epitope Tag Antibody

Sequenz: HHHHHH

Kreuzreaktivität (Details): This monoclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag.

Produktmerkmale: Synonyms: mouse anti-6X His Tag Antibody, anti-HIS, HIS Antibody, 6X His Tag Antibody, HHHHHH epitope tag antibody

Aufreinigung: 6X HIS Epitope Tag antibody was purified from concentrated tissue culture supernate by Protein A chromatography and is directed against the 6X His motif and is useful in determining its presence in various assays.

Sterilität: Sterile filtered

Immunogen:

Immunogen: HIS Tag antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.

Immunogen Type: Conjugated Peptide

Klon: 33D10-D2-G8

Isotyp: IgG1 kappa

Produktspezifische Information:

Wofür kann der His-Tag-Antikörper ABIN964552 verwendet werden? Dieser His-tag-Antikörper ist ein monoklonaler, unkonjugierter Antikörper für den spezifischen Nachweis von Fusionsproteinen, die entweder amino- oder carboxyterminale His-Epitop-Tags enthalten. Er eignet sich für den Nachweis von His-tag in ELISA- und Western-Blot-, IHC- und IP-Experimenten.

Welche Validierungsdaten gibt es für diesen His-tag-Antikörper? Der His-tag-Antikörper wurde bisher in 4 Publikationen verwendet, die bei Pubmed eingesehen werden können. Vier Bilder sind für die Validierung des Antikörpers im Western Blotting und ELISA verfügbar.

Was ist die Funktion von His-Tag? Der His-Tag ist einer der am häufigsten verwendeten Marker. Es handelt sich um eine Reihe von sechs bis neun Histidinresten, die entweder an den Amino- oder Carboxylterminus eines rekombinanten Proteins fusioniert sind. Die His-Kennzeichnung ist besonders nützlich, weil sie eine einfache Reinigung und den Nachweis des Zielproteins ermöglicht. Die geringe Größe des His-Tags im Vergleich zu anderen üblichen Epitop-Tags macht es weniger wahrscheinlich, dass es die Struktur oder Funktion des Zielproteins beeinträchtigt. Daher ist er für die Verwendung unter denaturierenden Bedingungen gut geeignet. Verwenden Sie unseren hochwertigen His-tag-Antikörper, um His-tag zuverlässig nachzuweisen.

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. As such, Anti-6X His/6XHIS can be used to identify fusion proteins that contain the 6X His epitope.  The antibody recognizes the His tag fused either to the amino- or carboxy- termini of targeted proteins.  This antibody has been tested by ELISA and western blotting against both the immunizing peptide and His-containing recombinant proteins.  Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry.

Western Blot Dilution: 1:1000

Immunoprecipitation Dilution: 1:200

ELISA Dilution: 1:75,000

Other: FLUORESCENT POLARIZATION: 2 - 20 nMFRET: 2 - 20 nM

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 3.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store 6X HIS Tag Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Anti-6X HIS Tag is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-His Tag Antikörper (ABIN964552)

Zhou, Pu, Chen, Hao, Gao, Ali, Hsiao, Stock, Goulian, Zhu: "Thiol-based functional mimicry of phosphorylation of the two-component system response regulator ArcA promotes pathogenesis in enteric pathogens." in: Cell reports, Vol. 37, Issue 12, pp. 110147, (2022) (PubMed).

Vela-Corcía, Romero, de Vicente, Pérez-García: "Analysis of β-tubulin-carbendazim interaction reveals that binding site for MBC fungicides does not include residues involved in fungicide resistance." in: Scientific reports, Vol. 8, Issue 1, pp. 7161, (2019) (PubMed).

Chavda, Ling, Majernick, Planey: "Antiproliferative factor (APF) binds specifically to sites within the cytoskeleton-associated protein 4 (CKAP4) extracellular domain." in: BMC biochemistry, Vol. 18, Issue 1, pp. 13, (2017) (PubMed).

Ramón-García, Ng, Jensen, Dosanjh, Burian, Morris, Folcher, Eltis, Grzesiek, Nguyen, Thompson: "WhiB7, an Fe-S-dependent transcription factor that activates species-specific repertoires of drug resistance determinants in actinobacteria." in: The Journal of biological chemistry, Vol. 288, Issue 48, pp. 34514-28, (2014) (PubMed).

Details zu His Tag

Target: His Tag

Andere Bezeichnung: 6X His (His Tag Produkte)

Substanzklasse: Tag

Hintergrund: Background: 6X His Tag Antibody as well as other Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies.  Due to their small size, epitope tags do not affect the tagged protein's biochemical properties.   Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence.  This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means.  This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.  The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures.  Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.