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His Tag Antikörper (HRP)

WB, ELISA, IP Wirt: Maus Monoclonal 33D10-D2 HRP
Produktnummer ABIN964554
  • Target Alle His Tag Antikörper anzeigen
    His Tag
    Reaktivität
    Bitte anfragen
    Wirt
    • 91
    • 60
    • 11
    • 9
    • 5
    • 4
    • 1
    • 1
    Maus
    Klonalität
    • 93
    • 76
    • 10
    Monoklonal
    Konjugat
    • 79
    • 21
    • 13
    • 13
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser His Tag Antikörper ist konjugiert mit HRP
    Applikation
    • 140
    • 90
    • 38
    • 27
    • 25
    • 18
    • 15
    • 14
    • 14
    • 13
    • 13
    • 7
    • 7
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunoprecipitation (IP)
    Sequenz
    HHHHHH
    Spezifität
    This protein-A purified antibody is directed against the 6X His motif and is useful in determining its presence in various assays. This monoclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag.  To date, this antibody has reacted with all His tagged proteins so far tested.  In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.  The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy-termini of targeted proteins in transfected or transformed cells.
    Produktmerkmale
    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often, sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag-containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
    Sterilität
    Sterile filtered
    Immunogen
    This antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
    Immunogen Type: Peptide
    Klon
    33D10-D2
    Isotyp
    IgG1 kappa
    Top Product
    Discover our top product His Tag Primärantikörper
  • Applikationshinweise
    Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. As such, anti-6X His/6X His can be used to identify fusion proteins containing the 6X His epitope.  The antibody recognizes the His tag fused either to the amino- or carboxy- termini of targeted proteins.  This antibody has been tested by ELISA and western blotting against both the immunizing peptide and His-containing recombinant proteins.  Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL
    Konzentration
    1.0 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
    Konservierungsmittel
    Gentamicin sulfate
    Handhabung
    Do NOT add Sodium Azide!
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
    Haltbarkeit
    12 months
  • Li, Xu, Wang, Gong, Liu, Zheng, Li, Li: "Epitope mapping reveals the binding mechanism of a functional antibody cross-reactive to both human and murine programmed death 1." in: mAbs, Vol. 9, Issue 4, pp. 628-637, (2018) (PubMed).

  • Target
    His Tag
    Abstract
    His Tag Produkte
    Substanzklasse
    Tag
    Hintergrund
    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies.  Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties.  Most often, sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence.  This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag-containing protein produced by recombinant means.  This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.  The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures.  Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.  Supplier produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG and HA. Supplier also produces antibodies to other tags including FITC, Rhodamine (TRITC), DNP and biotin.
    Synonyms: HRP anti-HIS, HIS Antibody, 6X His Tag Antibody, HHHHHH epitope tag antibody
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