alpha Tubulin Antikörper (C-Term)

Produktnummer ABIN964563

Produktdetails anti-alpha Tubulin Antikörper

Target: alpha Tubulin (TUBA1)

Bindungsspezifität: C-Term

Reaktivität: Human, Maus, Ratte, Huhn, Rind (Kuh)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser alpha Tubulin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Spezifität: Anti-Tubulin Loading Control Antibody was purified Protein A chromatography. This Loading Control antibody is directed against alpha tubulin.  A BLAST analysis was used to suggest antibody reactivity with alpha tubulin from a wide range of organisms, including avian, mammalian aquatic, parasitic and alga sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all isoforms of alpha tubulin. Such broad reactivity makes this antibody useful as an excellent loading control.

Kreuzreaktivität: Maus, Ratte (Rattus), Huhn, Schaf

Produktmerkmale: Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle. This antibody makes an excellent loading control.

Sterilität: Sterile filtered

Immunogen: Anti-Tubulin Loading Control Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues near the C terminal end of of human alpha tubulin protein.

Klon: 17H11-F10

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Anti-Tubulin Antibody has been tested for use in ELISA, immunohistochemistry, immunofluorescence microscopy and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band at ~50 kDa in size corresponding to alpha tubulin by western blotting in most cell lysates or extracts.

Kommentare:

Gene Name: TUBA1B

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Haltbarkeit: 12 months

Referenzen für anti-alpha Tubulin Antikörper (ABIN964563)

Clayton, Ban, Liu, Serra: "Canonical and noncanonical TGF-β signaling regulate fibrous tissue differentiation in the axial skeleton." in: Scientific reports, Vol. 10, Issue 1, pp. 21364, (2021) (PubMed).

Chavez, Coricor, Perez, Seo, Serra: "SOX9 protein is stabilized by TGF-β and regulates PAPSS2 mRNA expression in chondrocytes." in: Osteoarthritis and cartilage, Vol. 25, Issue 2, pp. 332-340, (2018) (PubMed).

Patel, Blaauboer, Tucker, Mansouri, Ruiz-Moreno, Hamann, Schumann, Opitz, Jin: "The Common R71H-G230A-R293Q Human TMEM173 Is a Null Allele." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 198, Issue 2, pp. 776-787, (2017) (PubMed).

Roy, Jerman, Jozsef, McNamara, Onyekaba, Sun, Marin: "Palmitoylation of the ciliary GTPase ARL13b is necessary for its stability and its role in cilia formation." in: The Journal of biological chemistry, Vol. 292, Issue 43, pp. 17703-17717, (2017) (PubMed).

Laitem, Zaborowska, Isa, Kufs, Dienstbier, Murphy: "CDK9 inhibitors define elongation checkpoints at both ends of RNA polymerase II-transcribed genes." in: Nature structural & molecular biology, Vol. 22, Issue 5, pp. 396-403, (2015) (PubMed).

Blaauboer, Gabrielle, Jin: "MPYS/STING-mediated TNF-α, not type I IFN, is essential for the mucosal adjuvant activity of (3'-5')-cyclic-di-guanosine-monophosphate in vivo." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 192, Issue 1, pp. 492-502, (2014) (PubMed).

Details zu alpha Tubulin

Target: alpha Tubulin (TUBA1)

Andere Bezeichnung: Alpha-Tubulin (TUBA1 Produkte)

Synonyme: Tuba1 antikoerper, K-ALPHA-1 antikoerper, Tuba-1 antikoerper, cb944 antikoerper, fb22g06 antikoerper, tuba1 antikoerper, wu:fb22g06 antikoerper, Calpha1 antikoerper, tubulin, alpha 1A antikoerper, tubulin alpha 1b antikoerper, tubulin, alpha 1b antikoerper, tubulin, alpha 1B antikoerper, Tuba1a antikoerper, TUBA1B antikoerper, tuba1b antikoerper, Tuba1b antikoerper

Hintergrund: Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle.  This antibody makes an excellent loading control.
Synonyms: Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain, Alpha-tubulin ubiquitous Tubulin K-alpha-1, TUBA1B, tubulin loading control

Gen-ID: 17986283

UniProt: P68363

Pathways: Microtubule Dynamics