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Biotin Antikörper

Rockland 600-401-098 WB, ELISA, IHC Wirt: Kaninchen Polyclonal unconjugated
Rockland
Produktnummer ABIN964580
Hersteller Produkt- Nr.: 600-401-098
  • Target Alle Biotin Produkte
    Biotin
    Reaktivität
    Bitte anfragen
    Wirt
    • 63
    • 36
    • 28
    Kaninchen
    Klonalität
    • 81
    • 38
    • 1
    Polyklonal
    Konjugat
    • 32
    • 13
    • 8
    • 8
    • 8
    • 6
    • 5
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser Biotin Antikörper ist unkonjugiert
    Applikation
    • 75
    • 52
    • 50
    • 46
    • 39
    • 32
    • 16
    • 13
    • 11
    • 9
    • 6
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
    Hersteller Produkt- Nr.
    600-401-098
    Hersteller
    Rockland
    Verwendungszweck
    Biotin Antibody
    Kreuzreaktivität (Details)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Biotin conjugated IgG and Biotin conjugated Bovine Serum Albumin.
    Produktmerkmale
    Synonyms: rabbit anti Biotin Antibody, rabbit anti-biotin Antibody
    Aufreinigung
    Biotin Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads.
    Sterilität
    Sterile filtered
    Immunogen

    Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)

    Immunogen Type: Other

    Isotyp
    IgG
  • Applikationshinweise

    Immunohistochemistry Dilution: User Optimized

    Application Note: Anti-Biotin Antibody has been tested by ELISA and is suitable for immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.

    Western Blot Dilution: 1:2,000 - 1:10,000

    ELISA Dilution: 1:200,000

    Other: User Optimized

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1.02 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    This product is stable for several weeks at 4° C as an undiluted liquid. Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. 
    Haltbarkeit
    12 months
  • Goulielmaki, Ioannidou, Tsekrekou, Stratigi, Poutakidou, Gkirtzimanaki, Aivaliotis, Evangelou, Topalis, Altmüller, Gorgoulis, Chatzinikolaou, Garinis: "Tissue-infiltrating macrophages mediate an exosome-based metabolic reprogramming upon DNA damage." in: Nature communications, Vol. 11, Issue 1, pp. 42, (2020) (PubMed).

  • Target
    Biotin
    Abstract
    Biotin Produkte
    Substanzklasse
    Chemical
    Hintergrund
    Background: Biotin Antibody detects Biotin. Biotin is a water-soluble B-complex vitamin (vitamin B7). It is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis. Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids. Anti-Biotin Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research.
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