Crasp-2 Antikörper

Produktnummer ABIN964612

Produktdetails anti-Crasp-2 Antikörper

Target: Crasp-2 (Complement regulator-acquiring surface protein 2 (Crasp-2))

Reaktivität: Borrelia burgdorferi

Wirt: Kaninchen

Klonalität: Polyklonal

Applikation: Lateral Flow (LF), Western Blotting (WB)

Spezifität: This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography. This antibody is specific for Borrelia burgdorferi CRASP-2 protein. A BLAST analysis was used to suggest reactivity with CRASP-2 from B. burgdorferi sources based on 100% homology with the immunizing sequence. Partial cross-reactivity is expected against B. garinii, B. spielmanii, and valaisiana sources based on 91-89% homology. Cross-reactivity with CRASP-2 from other sources has not been determined.

Produktmerkmale: CRASP-2 (Complement Regulator-Acquiring Surface Protein 2) of Borrelia burgdorferi binds FHL-1 and factor H binding protein in a distinct way. It may be predominantly expressed by serum-resistant Borrelia strains. Borrelia burgdorferi sensu lato has the ability to evade immune systems to persist in a variety of vertebrate hosts. This activity is dependent on a number of factors. Some Borrelia species bind host-derived fluid-phase immune regulators FHL-1 and factor H to their surface via complement regulator-acquiring surface proteins (CRASPs). Factor H and FHL-1 serve as cofactors for factor I, a serine protease that cleaves complement component 3b (C3b) directly on the cell surface and thereby confers resistance of spirochetes to complement-mediated lysis. It is possible that because of discontinuous binding regions in the factor H/FHL-1, long distance interaction may be involved in binding of both immune regulators. Putative coiled-coil structural elements may be important in the interaction of B. burgdorferi CRASP-1 with factor H.

Aufreinigung: purified

Sterilität: Sterile filtered

Immunogen: MBP-fusion protein corresponding to Borrelia burgdorferi CRASP-2 protein.
Immunogen Type: RecombinantProtein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: This protein-A purified antibody has been tested for use in Western blotting. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 25.4 kDa in size corresponding to Borrelia burgdorferi CRASP-2 protein by Western blotting in the appropriate cell lysate or extract.

Kommentare:

Gene Name: cspZ, BB_H06

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Haltbarkeit: 12 months

Details zu Crasp-2

Target: Crasp-2 (Complement regulator-acquiring surface protein 2 (Crasp-2))

Andere Bezeichnung: CRASP-2 (Crasp-2 Produkte)

Synonyme: complement regulator-acquiring surface protein antikoerper, BB_H06 antikoerper

Hintergrund: CRASP-2 (Complement Regulator-Acquiring Surface Protein 2) of Borrelia burgdorferi binds FHL-1 and factor H binding protein in a distinct way. It may be predominantly expressed by serum-resistant Borrelia strains. Borrelia burgdorferi sensu lato has the ability to evade immune systems to persist in a variety of vertebrate hosts. This activity is dependent on a number of factors. Some Borrelia species bind host-derived fluid-phase immune regulators FHL-1 and factor H to their surface via complement regulator-acquiring surface proteins (CRASPs). Factor H and FHL-1 serve as cofactors for factor I, a serine protease that cleaves complement component 3b (C3b) directly on the cell surface and thereby confers resistance of spirochetes to complement-mediated lysis. It is possible that because of discontinuous binding regions in the factor H/FHL-1, long distance interaction may be involved in binding of both immune regulators. Putative coiled-coil structural elements may be important in the interaction of B. burgdorferi CRASP-1 with factor H.
Synonyms: Borrelia burgdorferi CRASP-2

Gen-ID: 1194149

NCBI Accession: NP_045500

UniProt: O50665