ESRP1 Antikörper

Produktnummer ABIN964639

Produktdetails anti-ESRP1 Antikörper

Target: ESRP1 (Epithelial Splicing Regulatory Protein 1 (ESRP1))

Reaktivität: Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ESRP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Spezifität: This product is an IgG fraction antibody purified from tissue culture supernatant by Protein-A chromatography followed by extensive dialysis against the buffer stated above. This antibody reacts with mouse Esrp-1 protein.  A BLAST analysis of the immunizing protein sequence shows 100% homology with Esrp-1 from mouse and a 91% sequence homology with Esrp-1 from human, pig, rat, opossum, horse, cattle, panda, dog, and chimpanzee .  The binding epitope of this monoclonal antibody has not been mapped.

Produktmerkmale: Epithelial splicing regulatory protein 1 (Esrp-1) is an mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. It specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2, and also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Esrp-1 acts by directly binding specific sequences in mRNAs. It binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2.

Aufreinigung: purified

Sterilität: Sterile filtered

Immunogen: Anti-Esrp-1 was produced by repeated immunizations of full length recombinant mouse Esrp-1 fusion protein.
Immunogen Type: RecombinantProtein

Klon: 27H12-F3-H5

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: This protein-A purified antibody has been tested for use western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 75.5 kDa in size corresponding to Esrp-1 by western blotting in the appropriate cell lysate or extract.

Kommentare:

Gene Name: Esrp1

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is three (3) months from date of opening.

Haltbarkeit: 3 months

Referenzen für anti-ESRP1 Antikörper (ABIN964639)

Kim, Kim, Oh, Hong, Kim: "Pressure-overload cardiac hypertrophy is associated with distinct alternative splicing due to altered expression of splicing factors." in: Molecules and cells, Vol. 37, Issue 1, pp. 81-7, (2014) (PubMed).

Warzecha, Jiang, Amirikian, Dittmar, Lu, Shen, Guo, Xing, Carstens: "An ESRP-regulated splicing programme is abrogated during the epithelial-mesenchymal transition." in: The EMBO journal, Vol. 29, Issue 19, pp. 3286-300, (2010) (PubMed).

Details zu ESRP1

Target: ESRP1 (Epithelial Splicing Regulatory Protein 1 (ESRP1))

Andere Bezeichnung: Esrp-1 (ESRP1 Produkte)

Synonyme: rbm35a antikoerper, RBM35A antikoerper, RMB35A antikoerper, 2210008M09Rik antikoerper, A630065D16 antikoerper, BC031468 antikoerper, Rbm35a antikoerper, RGD1560481 antikoerper, wu:fi28a07 antikoerper, zgc:154050 antikoerper, epithelial splicing regulatory protein 1 antikoerper, epithelial splicing regulatory protein 1 L homeolog antikoerper, ESRP1 antikoerper, esrp1.L antikoerper, Esrp1 antikoerper, esrp1 antikoerper

Hintergrund: Epithelial splicing regulatory protein 1 (Esrp-1) is an mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. It specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2, and also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Esrp-1 acts by directly binding specific sequences in mRNAs. It binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2.
Synonyms: Rbm35a, Epithelial splicing regulatory protein 1, RNA-binding protein 35A, RNA-binding motif protein 35A.

Gen-ID: 207920

NCBI Accession: NP_918944

UniProt: Q3US41