ESRP1 Antikörper

Produktnummer ABIN964641

Hersteller Produkt- Nr.: 210-301-c31

Produktdetails anti-ESRP1 Antikörper

Target: ESRP1 (Epithelial Splicing Regulatory Protein 1 (ESRP1))

Reaktivität: Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ESRP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Hersteller Produkt- Nr.: 210-301-c31

Hersteller: Rockland

Verwendungszweck: Esrp-1/2 Antibody

Kreuzreaktivität (Details): This antibody reacts with both mouse Esrp-1 and Esrp-2 proteins.

Produktmerkmale: Synonyms: mouse anti-Esrp1/2 antibody, mouse anti-Esrp1 antibody, mouse anti-Ersp2 antibody, Rbm35a, Epithelial splicing regulatory protein 1, RNA-binding protein 35A, RNA-binding motif protein 35A, RNA-binding protein 35B, RNA-binding motif protein 35B

Aufreinigung: This antibody was purified from tissue culture supernatant by Protein-A chromatography followed by extensive dialysis against the buffer stated above.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Anti-Esrp-1/2 was produced by repeated immunizations of full length recombinant mouse Esrp-1 fusion protein.

Immunogen Type: Recombinant Protein

Klon: 23A7-C9

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

Application Note: This protein-A purified antibody has been tested for use western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 75.5 kDa in size corresponding to Esrp-1 and 77.4 kDa in size corresponding to Esrp-2 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:1000

ELISA Dilution: 1:750,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-ESRP1 Antikörper (ABIN964641)

Warzecha, Jiang, Amirikian, Dittmar, Lu, Shen, Guo, Xing, Carstens: "An ESRP-regulated splicing programme is abrogated during the epithelial-mesenchymal transition." in: The EMBO journal, Vol. 29, Issue 19, pp. 3286-300, (2010) (PubMed).

Details zu ESRP1

Target: ESRP1 (Epithelial Splicing Regulatory Protein 1 (ESRP1))

Andere Bezeichnung: Esrp1/Esrp2 (ESRP1 Produkte)

Hintergrund: Background: Epithelial splicing regulatory protein-1 (Esrp-1) and Esrp-2 are mRNA splicing factors that regulate the formation of epithelial cell-specific isoforms. They specifically regulate the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2, and also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Esrp-1 and -2 act by directly binding specific sequences in mRNAs. They bind the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2.

Gen-ID: 77411

NCBI Accession: NP_789808

UniProt: Q8K0G8