ESRP2 Antikörper

Produktnummer ABIN964643

Hersteller Produkt- Nr.: 210-301-c32

Produktdetails anti-ESRP2 Antikörper

Target: ESRP2 (Epithelial Splicing Regulatory Protein 2 (ESRP2))

Reaktivität: Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ESRP2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Hersteller Produkt- Nr.: 210-301-c32

Hersteller: Rockland

Verwendungszweck: Esrp-2 Antibody

Kreuzreaktivität (Details): This antibody reacts with mouse Esrp-2 protein.

Produktmerkmale: Synonyms: mouse anti-Esrp2 antibody, mouse anti-Esrp-2 antibody, RNA-binding protein 35B, RNA-binding motif protein 35B

Aufreinigung: Anti-Esrp-2 is an IgG fraction antibody purified from tissue culture supernatant by Protein-A chromatography followed by extensive dialysis against the buffer stated above.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Anti-Esrp-2 is produced by repeated immunizations of full length recombinant mouse Esrp-2 fusion protein.

Immunogen Type: Recombinant Protein

Klon: 22C5-H7-A6

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Esrp-2 protein A purified antibody has been tested for use western blotting and ELISA. This antibody shows specific reactivity by with ESRP2 transfected cell lysates.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 77.4 kDa in size corresponding to Esrp-2 by western blotting in the appropriate cell lysate or extract. The antibody shows bands at ~77kD and 70kD and no reactivity is observed in cells transfected with GFP or ESRP1. A starting dilution of 1:1000 is suggested for western blotting.

Western Blot Dilution: 1:1000

ELISA Dilution: 1:100,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-ESRP2 Antikörper (ABIN964643)

Heilmann, Toth, Bossmann, Klimo, Plass, Gerhauser: "Genome-wide screen for differentially methylated long noncoding RNAs identifies Esrp2 and lncRNA Esrp2-as regulated by enhancer DNA methylation with prognostic relevance for human breast cancer." in: Oncogene, Vol. 36, Issue 46, pp. 6446-6461, (2017) (PubMed).

Warzecha, Jiang, Amirikian, Dittmar, Lu, Shen, Guo, Xing, Carstens: "An ESRP-regulated splicing programme is abrogated during the epithelial-mesenchymal transition." in: The EMBO journal, Vol. 29, Issue 19, pp. 3286-300, (2010) (PubMed).

Details zu ESRP2

Target: ESRP2 (Epithelial Splicing Regulatory Protein 2 (ESRP2))

Andere Bezeichnung: Esrp2 (ESRP2 Produkte)

Hintergrund: Background: Epithelial splicing regulatory protein 2 (Esrp-2) is an mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. It specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2, and also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Esrp-2 acts by directly binding specific sequences in mRNAs. It binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2.

Gen-ID: 77411

UniProt: Q8K0G8