ESRP2 Antikörper

Produktnummer ABIN964643

Produktdetails anti-ESRP2 Antikörper

Target: ESRP2 (Epithelial Splicing Regulatory Protein 2 (ESRP2))

Reaktivität: Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ESRP2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Spezifität: This product is an IgG fraction antibody purified from tissue culture supernatant by Protein-A chromatography followed by extensive dialysis against the buffer stated above. This antibody reacts with mouse Esrp-2 protein.  A BLAST analysis was used to suggest cross-reactivity with Esrp-2 from mouse based on a 100% homology with the immunizing sequence.  Cross-reactivity with Esrp-2 from other sources has not been determined.

Produktmerkmale: Epithelial splicing regulatory protein 2 (Esrp-2) is an mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. It specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2, and also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Esrp-2 acts by directly binding specific sequences in mRNAs. It binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2.

Aufreinigung: purified

Sterilität: Sterile filtered

Immunogen: Anti-Esrp-2 is produced by repeated immunizations of full length recombinant mouse Esrp-2 fusion protein.
Immunogen Type: RecombinantProtein

Klon: 22C5-H7-A6

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Anti-Esrp-2 protein A purified antibody has been tested for use western blotting and shows specific reactivity by with ESRP2 transfected cell lysates..  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 77.4 kDa in size corresponding to Esrp-2 by western blotting in the appropriate cell lysate or extract. The antibody shows bands at ~77kD and 70kD and no reactivity is observed in cells transfected with GFP or ESRP1. A starting dilution of 1:1000 is suggested for western blotting.

Kommentare:

Gene Name: Esrp2

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Haltbarkeit: 12 months

Referenzen für anti-ESRP2 Antikörper (ABIN964643)

Heilmann, Toth, Bossmann, Klimo, Plass, Gerhauser: "Genome-wide screen for differentially methylated long noncoding RNAs identifies Esrp2 and lncRNA Esrp2-as regulated by enhancer DNA methylation with prognostic relevance for human breast cancer." in: Oncogene, Vol. 36, Issue 46, pp. 6446-6461, (2017) (PubMed).

Warzecha, Jiang, Amirikian, Dittmar, Lu, Shen, Guo, Xing, Carstens: "An ESRP-regulated splicing programme is abrogated during the epithelial-mesenchymal transition." in: The EMBO journal, Vol. 29, Issue 19, pp. 3286-300, (2010) (PubMed).

Details zu ESRP2

Target: ESRP2 (Epithelial Splicing Regulatory Protein 2 (ESRP2))

Andere Bezeichnung: Esrp-2 (ESRP2 Produkte)

Synonyme: RBM35B antikoerper, 9530027K23Rik antikoerper, Rbm35b antikoerper, RGD1310855 antikoerper, cb404 antikoerper, fa07a06 antikoerper, rbm35b antikoerper, sb:cb404 antikoerper, zgc:77254 antikoerper, epithelial splicing regulatory protein 2 antikoerper, microRNA 6773 antikoerper, ESRP2 antikoerper, Esrp2 antikoerper, MIR6773 antikoerper, esrp2 antikoerper

Hintergrund: Epithelial splicing regulatory protein 2 (Esrp-2) is an mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. It specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2, and also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Esrp-2 acts by directly binding specific sequences in mRNAs. It binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2.
Synonyms: RNA-binding protein 35B, RNA-binding motif protein 35B.

Gen-ID: 77411

NCBI Accession: NP_789808

UniProt: Q8K0G8