ZNF148 Antikörper
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- Target Alle ZNF148 Antikörper anzeigen
- ZNF148 (Zinc Finger Protein 148 (ZNF148))
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Reaktivität
- Human
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser ZNF148 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), ELISA
- Hersteller Produkt- Nr.
- 100-401-685
- Hersteller
- Rockland
- Verwendungszweck
- ZBP-89 Antibody
- Kreuzreaktivität (Details)
- This polyclonal antibody is specific for human ZBP-89.
- Produktmerkmale
- Synonyms: rabbit anti-ZBP-89 Antibody, Transcription factor ZBP89 antibody, Zinc finger DNA binding protein 89 antibody, Zinc finger protein 148 antibody, ZNF 148 antibody, Zinc finger protein 148, Transcription factor ZBP-89, ZNF148
- Aufreinigung
- Anti-ZBP89 antibody was prepared from monospecific antiserum by delipidation and defibrination.
- Sterilität
- Sterile filtered
- Immunogen
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Immunogen: Purified full length ZBP-89 recombinant protein expressed in E.coli.
Immunogen Type: Recombinant Protein
- Top Product
- Discover our top product ZNF148 Primärantikörper
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- Applikationshinweise
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Application Note: This polyclonal antibody reacts with human ZBP-89 in a variety of tested immunological assays including western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. For immunoblotting a 1:5,000 dilution is recommended. A band at approximately 89 kDa corresponding to human ZBP-89 is detected. Human monocytes or macrophages or nuclear extracts from PMA treated U937 cells can be used as a positive control. For ELISA a 1:10,000 to 1:30,000 dilution is recommended. Researchers should determine optimal titers for other applications.
Western Blot Dilution: 1:5,000
ELISA Dilution: 1:10,000 - 1:30,000
Other: User Optimized
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 90 mg/mL
- Buffer
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Buffer: None
Stabilizer: None
Preservative: 0.1 % (w/v) Sodium Azide - Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Store ZBP89 Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Haltbarkeit
- 12 months
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Characterization of a functional ZBP-89 binding site that mediates Gata1 gene expression during hematopoietic development." in: The Journal of biological chemistry, Vol. 284, Issue 44, pp. 30187-99, (2009) (PubMed).
: "Characterization of a megakaryocyte-specific enhancer of the key hemopoietic transcription factor GATA1." in: The Journal of biological chemistry, Vol. 281, Issue 19, pp. 13733-42, (2006) (PubMed).
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Characterization of a functional ZBP-89 binding site that mediates Gata1 gene expression during hematopoietic development." in: The Journal of biological chemistry, Vol. 284, Issue 44, pp. 30187-99, (2009) (PubMed).
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- Target
- ZNF148 (Zinc Finger Protein 148 (ZNF148))
- Andere Bezeichnung
- ZNF148 (ZNF148 Produkte)
- Hintergrund
- Background: The GI tract abundantly expresses growth factors many of which bind and activate the EGF receptor present on mucosal cells. One such factor is the zinc finger protein (ZBP-89) that binds to a GC-rich DNA element in the gastrin promoter and confers EGF responsiveness. The full-length protein functions as a repressor of growth factor signals regulating the gastrin promoter. Several other growth related promoters are also regulated by ZBP-89. ZBP-89 is one of a family of related transcriptional regulators. It has been reported in recent studies that ZBP-89 regulates growth in part by stimulating the cyclin-dependent kinase inhibitor, p21waf1, in a butyrate-dependent manner through recruitment of the histone acetyl transferase p300. Moreover, ZBP-89 triggers growth arrest in a p53-dependent manner by preventing nuclear export of p53. ZBP-89 also induces apoptosis, but this process occurs independent of p53.
- Gen-ID
- 256711
- UniProt
- Q9UQR1
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