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NF-kB p65 Antikörper (C-Term)

Name: NF-kB p65 Antikörper (C-Term)
Brand: antibodies-online
SKU: ABIN964680
Price: 623.7 EUR
Availability: InStock

NFkBP65 Reaktivität: Human WB, IHC, ELISA, IF, FM Wirt: Maus Monoclonal 27F9-G4 unconjugated

anti NFKB p65 (Rel A) monoclonal antibody was used to detect ~65 kD band (red arrow) in HeLa whole cell lysate. (NF-kB p65 Antikörper (C-Term))

Antibody has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. (NF-kB p65 Antikörper (C-Term))

IHC

Anti NFkB monoclonal antibody – Immunocytochemistry Tissue: Human Fibroblasts Top: Before activation Bottom: After activation with poly IC Left: 600-401-271 anti p65 NLS specific lot 18372 Right: Monoclonal antibody C-Term The two antibodies that are shown target different regions of the p65 protein. (NF-kB p65 Antikörper (C-Term))

Immunofluorescence (IF) image for anti-Nuclear Factor-kB p65 (NFkBP65) (C-Term) antibody (ABIN964680) (NF-kB p65 Antikörper (C-Term))

Monoclonal anti NFKB p65 (Rel A) antibody was used to detect p65 in b. (NF-kB p65 Antikörper (C-Term))

monoclonal anti p65 was used to detect p65 by Western blot. (NF-kB p65 Antikörper (C-Term))

6 Abbildungen

(2 Referenzen)

Produktnummer ABIN964680

Datenblatt (PDF)

Target Alle NF-kB p65 (NFkBP65) Antikörper anzeigen

NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))
Bindungsspezifität
37

31

28

26

23

22

22

19

19

16

15

15

13

9

9

6

6

6

5

5

5

5

4

4

4

4

4

4

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2
C-Term
Reaktivität
501

352

286

41

35

28

24

21

19

16

14

11

5

2

1

1

1

1
Human
Wirt
453

50

3

2

1
Maus
Passende Sekundärantikörper
Klonalität
435

74
Monoklonal
Konjugat
267

25

19

14

12

10

10

10

10

10

10

10

10

10

10

10

10

9

8

8

8

8

6

2

2

1
Dieser NF-kB p65 Antikörper ist unkonjugiert
Applikation
394

176

131

119

104

102

85

71

70

28

25

9

7

7

7

6

4

4

3

2

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM)

Verwendungszweck

NFkB p65 Antibody

Kreuzreaktivität (Details)

Reactivity was confirmed by ELISA against peptide conjugated carrier protein and by Western blot against HeLa whole cell lysate.

Produktmerkmale

Synonyms: mouse anti-NF-kB p65 Antibody, mouse anti-Rel A antibody, NFKB, nfkb, NF-kB, NF-kappaB, NFkappaB, Nuclear factor NF-kappa-B p65 subunit

Aufreinigung

Anti-NF-kB p65 Antibody was purified from concentrated tissue culture supernate by Protein A chromatography and showed a single band by IEP (immunoelectrophoresis) when tested with anti-mouse antibody.

Sterilität

Sterile filtered

Immunogen

Immunogen: NFkB p65 (Rel A) peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Immunogen Type: Conjugated Peptide

Klon

27F9-G4

Isotyp

IgG2a kappa

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anti-Nuclear Factor-kB p65 (NFkBP65) (AA 10-37), (N-Term) antibody
NFkBP65 Reaktivität: Human, Maus WB, IF, IHC (p), FACS Wirt: Kaninchen Polyclonal RB20786 unconjugated

anti-Nuclear Factor-kB p65 (NFkBP65) (pThr435) antibody
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anti-Nuclear Factor-kB p65 (NFkBP65) (pSer281) antibody
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Applikationshinweise

Immunohistochemistry Dilution: 1:200-1:600
Application Note: Anti-NF-kB p65 Antibody is a mouse monoclonal antibody directed against NFkB p65 (Rel A) and recognizes a 65 kD band by Western blot against HeLa whole cell lysate. Control peptide (100-4165p) is sold separately. This product tested in WB, ICC, IHC, and IF.
Western Blot Dilution: 1:1,000-1:5,000
ELISA Dilution: 1:50,000-1:100,000
IF Microscopy Dilution: 1:5,000
Other: User Optimized

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

1.0 mg/mL

Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Store NF-kB p65 Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit

12 months
Sikorski, Mehta, Inngjerdingen, Thakor, Kling, Kalina, Nyman, Stensland, Zhou, de Souza, Holden, Stuchly, Templin, Lund-Johansen: "A high-throughput pipeline for validation of antibodies." in: Nature methods, Vol. 15, Issue 11, pp. 909-912, (2019) (PubMed).

Merkhofer, Cogswell, Baldwin: "Her2 activates NF-kappaB and induces invasion through the canonical pathway involving IKKalpha." in: Oncogene, Vol. 29, Issue 8, pp. 1238-48, (2010) (PubMed).
Target

NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Andere Bezeichnung

RELA (NFkBP65 Produkte)

Hintergrund

Background: NFkappaB was originally identified as a factor that binds to the immunoglobulin kappa light chain enhancer in B cells. Other identified subunits include p52 (NFKB2), c-Rel, and RelB. The p65, cRel, and RelB subunits are responsible for transactivation. The p50 and p52 subunits possess DNA binding activity but limited ability to transactivate. p52 has been reported to form transcriptionally active heterodimers with the NFkappaB subunit p65, similar to p50/p65 heterodimers. The heterodimers of p52/p65 and p50/p65 are regulated by physical inactivation in the cytoplasm by IkappaBalpha. Cell Biology, Nuclear Signaling, Neuroscience and Signal Transduction Research.

Gen-ID

5970, 223468676

UniProt

Q04206

Pathways

NF-kappaB Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteine