LYZ Antikörper (AA 1-129)

Produktnummer ABIN964704

Hersteller Produkt- Nr.: 200-401-072

Dieses Anti-LYZ-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von LYZ in WB, IHC, IP, ELISA und DB. Geeignet für Huhn.

Kurzübersicht für LYZ Antikörper (AA 1-129) (ABIN964704)

Target: LYZ (Lysozyme (LYZ))

Reaktivität: Huhn

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LYZ Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), ELISA, Dot Blot (DB)

Produktdetails anti-LYZ Antikörper

Bindungsspezifität: AA 1-129

Hersteller Produkt- Nr.: 200-401-072

Hersteller: Rockland

Verwendungszweck: Lysozyme Antibody

Kreuzreaktivität (Details): This purified antibody is directed against lysozyme from hen egg white protein.

Produktmerkmale: Synonyms: rabbit anti-Lysozyme Antibody, 1 4 beta n acetylmuramidase c antibody, EC 3.2.1.17 antibody, LYZ antibody, Lysozyme C antibody, Lysozyme C precursor antibody, LZM antibody, Hen Egg White

Aufreinigung: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process, which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterilität: Sterile filtered

Immunogen:

Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length protein corresponding to amino acids 1-129 of Hen Egg White Lysozyme.

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:500 - 1:2,000

Application Note: Anti-Lysozyme Hen Egg White purified antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user.  Expect a band at ~14 kDa in size corresponding to lysozyme by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:2,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:25,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/ml

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu LYZ

Target: LYZ (Lysozyme (LYZ))

Andere Bezeichnung: LYZ

Hintergrund: Background: Lysozyme is a relatively small (129 AA) secretory enzyme that catalyzes the hydrolysis of β-1,4 glucosidic linkages between N-acetylmuramic acid (NAM) and N-acetylglucosamine (NAG) comprising the cell walls of bacteria and to a lesser degree chitin oligomers.  Lysozyme is common in animals and plants.  In birds, lysozyme is also an exceptionally abundant protein in egg whites.  Its biological function in fowl eggs is unclear.  Hen egg white lysozyme ''c'' is the most commonly studied form and source of the enzyme.  Lysozyme from domestic goose is designated lysozyme ''g''.  Lysozyme is also found in vertebrates, including human, mostly in secretions and certain tissues, such as saliva, tears, milk, cervical mucus, leucocytes, kidneys and urine, where it probably serves as an anti-bacterial agent by digesting and weakening the rigid bacterial cell wall, thereby rendering the bacteria susceptible to osmotic lysis.  Plant lysozyme is found in ficus and papaya latex, and is chemically distinct from the egg white enzyme.

Gen-ID: 396218

NCBI Accession: NP_990612

UniProt: P00698