Streptavidin Antikörper
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- Target Alle Streptavidin Antikörper anzeigen
- Streptavidin
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Reaktivität
- Streptomyces avidinii
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser Streptavidin Antikörper ist unkonjugiert
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Applikation
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Dot Blot (DB)
- Hersteller Produkt- Nr.
- 200-401-095
- Hersteller
- Rockland
- Verwendungszweck
- Streptavidin Antibody
- Kreuzreaktivität (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Streptavidin [Streptomyces avidinii].
- Produktmerkmale
- Synonyms: rabbit anti-Streptavidin Antibody, rabbit anti Streptavidin
- Aufreinigung
- Anti-Streptavidin antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- Sterilität
- Sterile filtered
- Immunogen
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Immunogen: Streptavidin [Streptomyces avidinii]
Immunogen Type: Native Protein
- Isotyp
- IgG
- Top Product
- Discover our top product Streptavidin Primärantikörper
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- Applikationshinweise
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Immunohistochemistry Dilution: 1:1,500 - 1:5,000
Application Note: Anti-Streptavidin antibody has been tested by ELISA and dot blot and is suitable for western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.
Western Blot Dilution: 1:3,000 - 1:12,000
ELISA Dilution: 1:200,000
Other: User Optimized
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Haltbarkeit
- 12 months
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- Target
- Streptavidin
- Abstract
- Streptavidin Produkte
- Hintergrund
- Background: Streptavidin antibody is a 60 kDa protein purified from the bacterium Streptomyces avidinii. Streptavidin homo-tetramers have an extraordinarily high affinity for biotin (also known as vitamin B7). With a dissociation constant (Kd) on the order of ~10-14 Mol/L,[1] the binding of biotin to streptavidin is one of the strongest non-covalent interactions known in nature. Streptavidin is used extensively in molecular biology and bio-nanotechnology due to the streptavidin-biotin complex's resistance to organic solvents, denaturants (e.g. guanidinium chloride), detergents (e.g. SDS, Triton), proteolytic enzymes, and extremes of temperature and pH . Anti-STREPTAVIDIN antibody is ideal for investigators involved in GFP and Epitope research.
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