OspC Antikörper

Produktnummer ABIN964717

Hersteller Produkt- Nr.: 200-401-c11

Produktdetails

Target: OspC

Reaktivität: Borrelia burgdorferi

Wirt: Kaninchen

Klonalität: Polyklonal

Applikation: ELISA, Western Blotting (WB)

Hersteller Produkt- Nr.: 200-401-c11

Hersteller: Rockland

Verwendungszweck: OspC Antibody

Kreuzreaktivität (Details): This antibody is specific for Borrelia burgdorferi OspC protein.

Produktmerkmale: Synonyms: rabbit anti-OspC Antibody, Borrelia burgdorferi OspC, PC, Outer Surface Protein C, BB_B19

Aufreinigung: This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography.

Immunogen:

Immunogen: MBP-fusion protein corresponding to Borrelia burgdorferi OspC protein.

Immunogen Type: Recombinant Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: This protein-A purified antibody has been tested for use in Western blotting and in ELISA. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 20.7 kDa in size corresponding to Borrelia burgdorferi OspC protein by Western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:1,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen

Karvonen, Tammisto, Nykky, Gilbert: "Borrelia burgdorferi Outer Membrane Vesicles Contain Antigenic Proteins, but Do Not Induce Cell Death in Human Cells." in: Microorganisms, Vol. 10, Issue 2, (2022) (PubMed).

Joung, Ballard, Ma, Tseng, Teshome, Burakowski, Garner, Di Carlo, Ozcan: "Paper-based multiplexed vertical flow assay for point-of-care testing." in: Lab on a chip, Vol. 19, Issue 6, pp. 1027-1034, (2019) (PubMed).

Caine, Lin, Kessler, Sato, Leong, Coburn: "Borrelia burgdorferi outer surface protein C (OspC) binds complement component C4b and confers bloodstream survival." in: Cellular microbiology, Vol. 19, Issue 12, (2018) (PubMed).

Caine, Coburn: "A short-term Borrelia burgdorferi infection model identifies tissue tropisms and bloodstream survival conferred by adhesion proteins." in: Infection and immunity, Vol. 83, Issue 8, pp. 3184-94, (2015) (PubMed).

Antigendetails

Target: OspC

Hintergrund: Background: Outer Surface Protein C, or OspC, is a 20.7 kDa immunogenic protein on the outer surface of the spirochete Borrelia burgdorferi. Its function is not known, but it is located with lipid-anchoring sites on the outer cell membrane.

Gen-ID: 1194415

NCBI Accession: WP_010890595

UniProt: Q07337