OspA Antikörper

Produktnummer ABIN964719

Produktdetails anti-OspA Antikörper

Target: OspA (Borrelia Burgdorferi Outer Surface Protein A (OspA))

Reaktivität: Borrelia burgdorferi

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser OspA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Lateral Flow (LF)

Spezifität: This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography. This antibody is specific for Borrelia burgdorferi OspA protein. A BLAST analysis was used to suggest cross-reactivity with OspA from B. burgdorferi and sources based on 100% homology with the immunizing sequence. Cross-reactivity with OspA or Osp from other sources has not been determined.

Produktmerkmale: Outer-Surface Protein A (OspA), a lipoprotein from Borrelia burgdorferi encoded on its Plasmid lp54, is a major component of the spirochete's extracellular matrix. OspA probably serves as a lipid-anchor. The spirochetes migrate from the tick midgut during feeding to its salivary glands and are thus transmitted to the mammal host. This transition may be facilitated by changes in expression of some B. burgdorferi genes. Upon transmission of the spirochete from the Ixodes tick to mammalian host, the transcript level of OspA can change. It is believed that expression of the various proteins associated with the spirochete may be regulated by the changes in tick life cycle, changes in conditions during tick feeding (such as temperature, pH, and nutrients) and/or in coordination with the course of infection of the mammal host. B. burgdorferi can attach to (and also differentially express antigens in) diverse tissues within the vertebrate host and the tick vector, suggesting that physiological factors other than pH and temperature may play roles in modulating B. burgdorferi gene expression.

Aufreinigung: purified

Sterilität: Sterile filtered

Immunogen: MBP-fusion protein corresponding to Borrelia burgdorferi OspA protein.
Immunogen Type: RecombinantProtein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: This protein-A purified antibody has been tested for use in Western blotting. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 28.1 kDa in size corresponding to Borrelia burgdorferi OspA protein by Western blotting in the appropriate cell lysate or extract.

Kommentare:

Gene Name: ospA, BB_A15

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening.

Haltbarkeit: 12 months

Details zu OspA

Target: OspA (Borrelia Burgdorferi Outer Surface Protein A (OspA))

Abstract: OspA Produkte

Hintergrund: Outer-Surface Protein A (OspA), a lipoprotein from Borrelia burgdorferi encoded on its Plasmid lp54, is a major component of the spirochete's extracellular matrix. OspA probably serves as a lipid-anchor. The spirochetes migrate from the tick midgut during feeding to its salivary glands and are thus transmitted to the mammal host. This transition may be facilitated by changes in expression of some B. burgdorferi genes. Upon transmission of the spirochete from the Ixodes tick to mammalian host, the transcript level of OspA can change. It is believed that expression of the various proteins associated with the spirochete may be regulated by the changes in tick life cycle, changes in conditions during tick feeding (such as temperature, pH, and nutrients) and/or in coordination with the course of infection of the mammal host. B. burgdorferi can attach to (and also differentially express antigens in) diverse tissues within the vertebrate host and the tick vector, suggesting that physiological factors other than pH and temperature may play roles in modulating B. burgdorferi gene expression.
Synonyms: Outer surface protein A, Borrelia burgdorferi OspA

Gen-ID: 1194357

NCBI Accession: NP_045688

UniProt: P0C926