IL-6 Antikörper
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- Target Alle IL-6 (IL6) Antikörper anzeigen
- IL-6 (IL6) (Interleukin 6 (IL6))
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Reaktivität
- Human
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser IL-6 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), ELISA, Flow Cytometry (FACS), Multiplex Assay (MA)
- Hersteller Produkt- Nr.
- 209-301-310
- Hersteller
- Rockland
- Verwendungszweck
- IL-6 Antibody
- Kreuzreaktivität (Details)
- This purified antibody detects recombinant and native IL-6 present in body fluids and cell supernatants in various assays (ie.IL-1 stimulated IL-6 production from fibroblasts).
- Produktmerkmale
- Synonyms: mouse Anti-IL-6 antibody, mouse anti-interleukin-6 antibody, HSF, Hybridoma growth factor, Hybridoma plasmacytoma growth factor, IFNB2, IL 6, IL6 protein, Interferon beta 2, Interleukin 6, BSF2, CDF
- Aufreinigung
- Protein A purified
- Sterilität
- Sterile filtered
- Endotoxin-Niveau
- Low Endotoxin
- Immunogen
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Immunogen: This Protein A purified IL-6 monoclonal antibody was produced in mouse by repeated immunizations with mature full length recombinant human IL-6 produced in E.coli followed by hybridoma development.
Immunogen Type: Recombinant Protein
- Klon
- 33A12-G9
- Isotyp
- IgG1 kappa
- Top Product
- Discover our top product IL6 Primärantikörper
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- Applikationshinweise
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Flow Cytometry Dilution: 0.5 mg/mL
Application Note: Anti-IL-6 antibody has been tested for use in ELISA, Flow Cytometry, and western blotting. Reactivity is also expected in neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/μl by the LAL method. By western blot a band approximately 23.7 kDa in size corresponding to native human IL-6 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: 1:1000
ELISA Dilution: 1:10,000
Other: User Optimized
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 2.19 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Store IL 6 antibody at -20° C . For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Haltbarkeit
- 12 months
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- Target
- IL-6 (IL6) (Interleukin 6 (IL6))
- Andere Bezeichnung
- IL6 (IL6 Produkte)
- Hintergrund
- Background: IL-6 is a secreted cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response and plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. IL-6 induces myeloma and plasmacytoma growth and induces nerve cells differentiation and acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. IL-6 also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. Anti-IL-6 antibody is ideal for investigators involved in Cancer, Neuroscience and Immunology research.
- Gen-ID
- 3569
- NCBI Accession
- NP_000591
- UniProt
- P05231
- Pathways
- TLR Signalweg, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagie, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome
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