VEGFA Antikörper (HRP)

Produktnummer ABIN964773

Hersteller Produkt- Nr.: 209-403-b99

Produktdetails anti-VEGFA Antikörper (HRP)

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser VEGFA Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Hersteller Produkt- Nr.: 209-403-b99

Hersteller: Rockland

Verwendungszweck: VEGF Antibody Peroxidase Conjugated

Kreuzreaktivität (Details): This purified antibody has been heated to 56 °C for 30 minutes.

Produktmerkmale: Synonyms: rabbit anti-VEGF Peroxidase Conjugated antibody, rabbit anti-VEGF-165 Peroxidase Conjugated antibody, Vascular endothelial growth factor A, VEGF-A, VEGF-165, VEGF165, VEGF isoform L, Vascular permeability factor, VPF

Aufreinigung: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human VEGF-165 protein.

Immunogen Type: Recombinant Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:500-1:2,500

Application Note: This protein-A purified antibody has been tested for use in western blotting. Reactivity in other assays has not been determined. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 22 kDa in size corresponding to monomeric human VEGF-165 protein by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:1,000-1:5,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-VEGFA Antikörper (HRP) (ABIN964773)

Sreevalsan, Safe: "The cannabinoid WIN 55,212-2 decreases specificity protein transcription factors and the oncogenic cap protein eIF4E in colon cancer cells." in: Molecular cancer therapeutics, Vol. 12, Issue 11, pp. 2483-93, (2014) (PubMed).

Details zu VEGFA

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Andere Bezeichnung: VEGFA (VEGFA Produkte)

Hintergrund: Background: VEGF (Vascular Endothelial Growth Factor A) is a homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. It exhibits potent mitogenic and permeability inducing properties specific for the vascular endothelium. Of the four isoforms of VEGF, the smaller two, VEGF 165 and VEGF 121, are secreted proteins and act as diffusible agents, whereas the larger two (VEGF 189 and VEGF 206) remain cell associated. The sequence of this isoform differs from the canonical sequence as follows: 141-141: K → N and 142-182: missing. This isoform is often found as a disulfide linked homodimer.

Gen-ID: 7422

NCBI Accession: NP_001165097

UniProt: P15692

Pathways: RTK Signalweg, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling