GST
Reaktivität: Diverse Spezies
WB, IP, DB
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
Western Blot: Dilution 1: 2000- 5,000 ELISA: Proposed dilution 1: 10,000. Determining optimal working dilutions by titration test.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Lagerung
-20 °C
Smith, Johnson: "Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase." in: Gene, Vol. 67, Issue 1, pp. 31-40, (1988) (PubMed).
Plasmid vectors for the expression of coding regions of eukaryotic genes in E. coliare in common usage, such expression vectors often encode hybrid fusion proteins containing part prokaryotic and part eukaryotic specified proteins. For instance, the pGEX.3X expression vector developed by Smith and Johnson allows for synthesis of fusion proteins between glutathionine-S-transferase (GST) and proteins encoded by inserted cDNA sequences. Antibodies derived from these GST fusion proteins are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in E. coli.