SEK1/MKK4 (Ab-261) antibody detects endogenous levels of total SEK1/MKK4 protein.
Aufreinigung
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human SEK1/MKK4 around the phosphorylation site of Threonine 261 (A-K-TP-R-D).
MAP2K4
Reaktivität: Human
ELISA, IHC (p), IF (cc), IF (p), IHC (fro)
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
IHC: 1:50-1:100.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
100ug/100ul.
Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
-20 °C
Wang, Chen, Liao, Deng, Zhou, Huang, Sun: "Sequential activation of the MEK-extracellular signal-regulated kinase and MKK3/6-p38 mitogen-activated protein kinase pathways mediates oncogenic ras-induced premature senescence." in: Molecular and cellular biology, Vol. 22, Issue 10, pp. 3389-403, (2002) (PubMed).
Leung, Lassam: "The kinase activation loop is the key to mixed lineage kinase-3 activation via both autophosphorylation and hematopoietic progenitor kinase 1 phosphorylation." in: The Journal of biological chemistry, Vol. 276, Issue 3, pp. 1961-7, (2001) (PubMed).
Target
MAP2K4
(Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4))