CD93 Antikörper

Produktnummer ABIN967346

Produktdetails anti-CD93 Antikörper

Target: CD93

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD93 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP)

Marke: BD Pharmingen™

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: C1q-binding protein

Klon: R3

Isotyp: IgM kappa

Anwendungsinformationen

Applikationshinweise: Immunofluorescent Staining and Flow Cytometric Analysis:
The staining technique and blocking controls are described in detail by C. Prussin and D. Metcalfe. A suitable mouse IgM, kappa isotype control for assessing the level of background staining on human cells is recommended: use at comparable concentrations to antibody of interest (eg, Less or equal than 0.5 µg Ab/1 million cells) (see Figure).

Western Blotting and immunoprecipitation:
When run under non-reducing conditions, Human C1qRp migrates as a 100 kDa protein, due to high level of glycosylation C1qR migrates as 126 kDa under reducing conditions. The R3 antibody is suitable to detect C1qRp by Western blotting and immunprecipitation as described, however, the antibody is not tested for this application. Reactivity of the antibody with the reduced protein is dramatically decreased.

Modulation of monocyte phagocytic activity:
Pretreatment of monocytes with R3 neutralizes C1q-mediated enhancement of phagocytosis. In addition, when immobilized, R3 mimics C1q- MBL- or SPA-mediated enhancement of phagocytosis, however, the antibody is not tested for this application.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-CD93 Antikörper (ABIN967346)

Danet, Luongo, Butler, Lu, Tenner, Simon, Bonnet: "C1qRp defines a new human stem cell population with hematopoietic and hepatic potential." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 16, pp. 10441-5, (2002) (PubMed).

Nepomuceno, Ruiz, Park, Tenner: "C1qRP is a heavily O-glycosylated cell surface protein involved in the regulation of phagocytic activity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 6, pp. 3583-9, (1999) (PubMed).

Tenner: "C1q receptors: regulating specific functions of phagocytic cells." in: Immunobiology, Vol. 199, Issue 2, pp. 250-64, (1999) (PubMed).

Nepomuceno, Tenner: "C1qRP, the C1q receptor that enhances phagocytosis, is detected specifically in human cells of myeloid lineage, endothelial cells, and platelets." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 4, pp. 1929-35, (1998) (PubMed).

Nepomuceno, Henschen-Edman, Burgess, Tenner: "cDNA cloning and primary structure analysis of C1qR(P), the human C1q/MBL/SPA receptor that mediates enhanced phagocytosis in vitro." in: Immunity, Vol. 6, Issue 2, pp. 119-29, (1997) (PubMed).

Guan, Robinson, Goodman, Tenner: "Cell-surface protein identified on phagocytic cells modulates the C1q-mediated enhancement of phagocytosis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 8, pp. 4005-16, (1994) (PubMed).

Guan, Burgess, Robinson, Goodman, McTigue, Tenner: "Phagocytic cell molecules that bind the collagen-like region of C1q. Involvement in the C1q-mediated enhancement of phagocytosis." in: The Journal of biological chemistry, Vol. 266, Issue 30, pp. 20345-55, (1991) (PubMed).

Details zu CD93

Target: CD93

Andere Bezeichnung: CDw93 (CD93 Produkte)

Synonyme: 6030404G09Rik antikoerper, AA145088 antikoerper, AA4.1 antikoerper, AW555904 antikoerper, C1qr1 antikoerper, C1qrp antikoerper, Ly68 antikoerper, C1QR1 antikoerper, C1qR(P) antikoerper, C1qRP antikoerper, CDw93 antikoerper, ECSM3 antikoerper, MXRA4 antikoerper, dJ737E23.1 antikoerper, C1qRp antikoerper, CD93 antigen antikoerper, CD93 molecule antikoerper, Cd93 antikoerper, CD93 antikoerper

Hintergrund: The immunogen used to raise R3 was C1q-binding protein preparation as described. Human C1qRp is a 631 a.a. protein (~66.5 kD) protein that is highly expressed on monocytes/macrophages, neutrophil granulocytes but not on T and B lymphocytes. C1qRp binds C1q, the recognition subunit of the first component (C1) of the complement pathway, as well as MBL (Mannose-binding-lectin) and SPA (Pulmonary Surfactant Protein A). Human C1qRp is involved in the C1q-mediated enhancement of phagocytosis. R3 is suitable to detect C1qRp expression on cells of myeloid lineage by flow cytometry, C1qRp in cellular lysates by Western blotting or immunoprecipitation. Pretreatment of monocytes with R3 neutralizes C1q-mediated enhancement of phagocytosis. In addition, when immobilized, R3 mimics C1q- MBL- or SPA-mediated enhancement of phagocytosis as reported.
CDw93 (C1qRp) has been reported to define a human stem cell population with hematopoietic and hepatic potential.
Synonyms: C1qRp