1. Since applications vary, each investigator should titrate the reagent to obtain optimal results. 2. Please refer to us for technical protocols. 3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. 4. Sodium azide is a reversible inhibitor of oxidative metabolism, therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
Aufreinigung
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen
Tumor-associated antigen TSP-180 from a BALB/c mouse lung carcinoma
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C
Informationen zur Lagerung
Store undiluted at 4° C.
Morena, Riccioni, Marchetti, Polcini, Mercurio, Blandino, Sacchi, Falcioni: "Expression of the beta 4 integrin subunit induces monocytic differentiation of 32D/v-Abl cells." in: Blood, Vol. 100, Issue 1, pp. 96-106, (2002) (PubMed).
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The 346-11A antibody reacts with an epitope at the beginning of the cysteine-rich repeat region of the 200-kDa integrin beta4 chain (CD104), which is found on the cell surface as a heterodimeric complex with the integrin alpha6 chain (CD49f). The alpha6beta4 complex binds to laminins and is expressed on the basal surface of a variety of epithelial cell types, particularly on stratified squamous epithelia, and is also found in peripheral nerves, in certain subsets of endothelial cells, and on immature thymocytes. It has also been identified on a number of tumor tissues and participates in tumor progression events. Because of the localization of both human and mouse epidermal alpha6beta4 integrin to hemidesmosomes, a role in epidermal adhesion to basement membrane has been suggested. Synonyms: Integrin beta4 chain