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E2F1 Antikörper

E2F1 Reaktivität: Human, Maus, Ratte WB, IP Wirt: Maus Monoclonal KH95-E2F unconjugated
Produktnummer ABIN967439
  • Target Alle E2F1 Antikörper anzeigen
    E2F1 (E2F Transcription Factor 1 (E2F1))
    Reaktivität
    • 207
    • 100
    • 54
    • 6
    • 6
    • 5
    • 5
    • 5
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    Human, Maus, Ratte
    Wirt
    • 210
    • 12
    • 2
    Maus
    Klonalität
    • 202
    • 22
    Monoklonal
    Konjugat
    • 93
    • 17
    • 17
    • 13
    • 10
    • 7
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    Dieser E2F1 Antikörper ist unkonjugiert
    Applikation
    • 188
    • 100
    • 67
    • 66
    • 51
    • 30
    • 22
    • 19
    • 18
    • 17
    • 15
    • 12
    • 6
    • 2
    • 1
    Western Blotting (WB), Immunoprecipitation (IP)
    Marke
    BD Pharmingen™
    Produktmerkmale
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    Aufreinigung
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Klon
    KH95-E2F
    Isotyp
    IgG2a
    Top Product
    Discover our top product E2F1 Primärantikörper
  • Applikationshinweise
    Applications include western blot analysis (dilute antibody 1-2 µg/ml). MOLT-4 leukemia cells (ATCC CRL-1582), Daudi lymphoma cells (ATCC CCL-213), and RS4, 11 leukemia cells (ATCC CRL-1873) are suggested as positive controls. Other applications include immunoprecipitation (1-2 µg/1x10^6 cells).
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    0.5 mg/mL
    Buffer
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Store undiluted at 4°C.
  • Saito, Helin, Valentine, Griffith, Willman, Harlow, Look: "Amplification of the E2F1 transcription factor gene in the HEL erythroleukemia cell line." in: Genomics, Vol. 25, Issue 1, pp. 130-8, (1995) (PubMed).

    Dyson, Dembski, Fattaey, Ngwu, Ewen, Helin: "Analysis of p107-associated proteins: p107 associates with a form of E2F that differs from pRB-associated E2F-1." in: Journal of virology, Vol. 67, Issue 12, pp. 7641-7, (1993) (PubMed).

    Helin, Wu, Fattaey, Lees, Dynlacht, Ngwu, Harlow: "Heterodimerization of the transcription factors E2F-1 and DP-1 leads to cooperative trans-activation." in: Genes & development, Vol. 7, Issue 10, pp. 1850-61, (1993) (PubMed).

    Huber, Edwards, Goodhart, Patrick, Huang, Ivey-Hoyle, Barnett, Oliff, Heimbrook: "Transcription factor E2F binds DNA as a heterodimer." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 8, pp. 3525-9, (1993) (PubMed).

    Nevins: "E2F: a link between the Rb tumor suppressor protein and viral oncoproteins." in: Science (New York, N.Y.), Vol. 258, Issue 5081, pp. 424-9, (1992) (PubMed).

  • Target
    E2F1 (E2F Transcription Factor 1 (E2F1))
    Andere Bezeichnung
    E2F-1 (E2F1 Produkte)
    Synonyme
    CG6376 antikoerper, DRTF1/E2F antikoerper, DmE2F-1 antikoerper, Dmel\\CG6376 antikoerper, Dp antikoerper, E(Sev-CycE)3A antikoerper, E(var)3-93E antikoerper, E(var)3-95E antikoerper, E(var)93E antikoerper, E2F antikoerper, E2F-1 antikoerper, E2F1 antikoerper, E2f1 antikoerper, Evar(3)164 antikoerper, dE2F antikoerper, dE2F1 antikoerper, dE2f antikoerper, dE2f1 antikoerper, de2f1 antikoerper, def21 antikoerper, drosE2F1 antikoerper, e2f antikoerper, e2f1 antikoerper, l(3)07172 antikoerper, l(3)j3B1 antikoerper, l(3)j3C2 antikoerper, l(3)rM729 antikoerper, RBAP1 antikoerper, RBBP3 antikoerper, RBP3 antikoerper, mKIAA4009 antikoerper, xE2F antikoerper, ATE2FB antikoerper, E2F TRANSCRIPTION FACTOR-1 E2F1 antikoerper, E2F transcription factor 1 antikoerper, E2FB antikoerper, E2FB TRANSCRIPTION FACTOR antikoerper, E2F transcription factor 1 antikoerper, E2F transcription factor 1 L homeolog antikoerper, E2f1 antikoerper, E2F1 antikoerper, e2f1.L antikoerper
    Hintergrund
    The transcription factor E2F was originally characterized as a sequence-specific DNA-binding factor bound to the adenovirus E2A promoter. E2F-binding sites have now been identified in cellular promoters for genes involved in growth regulation, including c-myc, N-myc, Cdc2 and cyclin A. They have also been identified in promoters for genes whose products are required for DNA synthesis and replication, such as DNA polymerase a, thymidine kinase, thymidylate synthase, and dihydrofolate reductase. When bound to DNA, E2F has been found in complexes with key regulators of cell proliferation including the retinoblastoma protein (Rb) and the Rb related proteins, p107 and p130. Like a variety of other transcription factors, E2F binds to DNA as a heterodimer. The protein E2F-1 has E2F-like properties, it mediates E2F-dependent trans-activation and binds to underphosphorylated Rb. E2F dimerizes with DP-1, a protein which also has E2F-like properties. This dimerization leads to increased DNA binding activity and enhanced transcriptional activity. It is thought that E2F-1/DP association forms an active E2F” transcription factor. E2F-1 migrates at a reduced molecular weight of ~60 kDa. Clone KH95/E2F (also referred to as KH95) recognizes an epitope between amino acids 342 and 386 of human E2F-1. It cross reacts with mouse and rat E2F-1. Full length, bacterially expressed, recombinant histidine-tagged human E2F-1 (amino acids 1-437) was purified and used as immunogen. Positive tissue culture supernatants were identified by immunoprecipitation of [35S]methionine-labeled, in vitro synthesized E2F-1 protein.
    Molekulargewicht
    60 kDa
    Pathways
    p53 Signalweg, Zellzyklus, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagie
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