HSP70 Antikörper

Produktnummer ABIN967447

Produktdetails anti-HSP70 Antikörper

Target: HSP70 (Heat Shock Protein 70 (HSP70))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HSP70 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Marke: BD Pharmingen™

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Purified Hsp70

Klon: 5G10

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: HeLa cervical carcinoma cells (ATCC CCL-2) are suggested as a positive control.

Kommentare:

Related Products: ABIN968535, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-HSP70 Antikörper (ABIN967447)

Cyr, Langer, Douglas: "DnaJ-like proteins: molecular chaperones and specific regulators of Hsp70." in: Trends in biochemical sciences, Vol. 19, Issue 4, pp. 176-81, (1994) (PubMed).

Craig, Gross: "Is hsp70 the cellular thermometer?" in: Trends in biochemical sciences, Vol. 16, Issue 4, pp. 135-40, (1991) (PubMed).

Singh, Yu: "Accumulation of a heat shock-like protein during differentiation of human erythroid cell line K562." in: Nature, Vol. 309, Issue 5969, pp. 631-3, (1984) (PubMed).

Details zu HSP70

Target: HSP70 (Heat Shock Protein 70 (HSP70))

Andere Bezeichnung: Hsp70 (HSP70 Produkte)

Hintergrund: Cells from many organisms increase the expression of heat shock or stress proteins in response to adverse changes. The heat shock protein (HSP) 70 family consists of multiple members, localized within different intracellular compartments. HSP 70 is constitutively expressed in many human cells with increased expression observed following stress. Members of the HSP 70 family bind ATP, and are thought to function as molecular chaperones by assisting in the folding of other proteins in various intracellular compartments. HSP 70 migrates at a molecular weight of 70 kDa in SDS-PAGE. The 5G10 antibody recognizes human HSP 70. Purified HSP 70 was isolated from K562, a human erythroid cell line, and used as immunogen.

Molekulargewicht: 70 kDa