CAN Antikörper (AA 349-505)

Produktnummer ABIN967510

Produktdetails anti-CAN Antikörper

Target: CAN (Calcineurin (CAN))

Bindungsspezifität: AA 349-505

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CAN Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Marke: BD Pharmingen™

Kreuzreaktivität: Human

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human beta1 isoform of calcineurin A Subunit aa. 349-505

Klon: G182-1847

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Applications include immunoprecipitation (1-2 µg/ml) and western blot analysis (1-2 µg/ml). Jurkat human T cells (ATCC TIB-152)2, HeLa human carcinoma cells (ATCC CCL-2), and A-431 human epidermal carcinoma cells (ATCC CRL-1555) are suggested as positive controls. A431 cell lysate (ABIN968533) and HeLa cell lysate (ABIN968535) are also available as ready-to-use positive western blot control.

Kommentare:

Related Products: ABIN967389, ABIN968533, ABIN968535

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-CAN Antikörper (ABIN967510)

Lai, Burnett, Wolosker, Blackshaw, Snyder: "Cain, a novel physiologic protein inhibitor of calcineurin." in: The Journal of biological chemistry, Vol. 273, Issue 29, pp. 18325-31, (1998) (PubMed).

Husi, Luyten, Zurini: "Mapping of the immunophilin-immunosuppressant site of interaction on calcineurin." in: The Journal of biological chemistry, Vol. 269, Issue 19, pp. 14199-204, (1994) (PubMed).

Fruman, Klee, Bierer, Burakoff: "Calcineurin phosphatase activity in T lymphocytes is inhibited by FK 506 and cyclosporin A." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 9, pp. 3686-90, (1992) (PubMed).

Details zu CAN

Target: CAN (Calcineurin (CAN))

Andere Bezeichnung: Calcineurin (CAN Produkte)

Synonyme: CALN antikoerper, CALNA antikoerper, CALNA1 antikoerper, CCN1 antikoerper, CNA1 antikoerper, PPP2B antikoerper, CAN A1/PP2B antikoerper, CG1455 antikoerper, Can antikoerper, Cana1 antikoerper, CnA antikoerper, CnnA21EF antikoerper, D14 antikoerper, DmCanA1-100B antikoerper, Dmel\\CG1455 antikoerper, PP2B antikoerper, PP2B 21EF antikoerper, PpD14 antikoerper, canA antikoerper, pMY4 antikoerper, 2900074D19Rik antikoerper, AI841391 antikoerper, AW413465 antikoerper, CN antikoerper, Caln antikoerper, Calna antikoerper, Calna1 antikoerper, protein phosphatase 3 catalytic subunit alpha antikoerper, Calcineurin A1 antikoerper, protein phosphatase 3, catalytic subunit, alpha isoform antikoerper, PPP3CA antikoerper, CanA1 antikoerper, Ppp3ca antikoerper

Hintergrund: Calcineurin is a Ca2+- and calmodulin-dependent serine/threonine phosphatase which consists of two subunits. The A subunit (~61 kDa) is catalytic and has calmodulinbinding activity, the B subunit (~19 kDa), which is myristylated at its N-terminus, has Ca[2+]-binding activity. Calcineurin is expressed ubiquitously in eukaryotic cells, including yeast. The protein is highly expressed in mammalian brain, and has been implicated in a number of calcium-dependent pathways in the nervous system, including regulation of neurotransmitter release. Calcineurin also plays a key role in T cell receptor-mediated signal transduction pathways. Calcineurin dephosphorylates components of NF-AT (Nuclear Factor of Activated T cells), allowing NF-AT to translocate to the nucleus where it participates with AP-1 in activation of multiple cytokine and surface receptor genes. Inhibition of calcineurin by immunosuppressive agents such as cyclosporin A and FK506 correlates with the suppression of early T cell activation events. These immunosuppressive agents bind to specific intracellular proteins, called immunophilins, to form complexes that inhibit calcineurin’s phosphatase activity. In the presence of these complexes, NF-AT remains phosphorylated and thus cannot translocate to the nucleus. A novel protein, Cain (calcineurin inhibitor), has been described which displays a similar pattern of neuronal and brain expression and has been shown to bind and inhibit calcineurin activity in vivo.
G182-1847 recognizes the alpha and beta isoforms of the A subunit of calcineurin (~61 kDa). It reacts with an epitope in the autoinhibitory domain. It does not recognize the B subunit of calcineurin. A recombinant tag-fusion protein containing amino acids 349-505 of the human beta1 isoform of calcineurin A subunit was used as immunogen.

Molekulargewicht: 61 kDa