CAN Antikörper (AA 349-505)

Produktnummer ABIN967510

Produktdetails anti-CAN Antikörper

Target: CAN (Calcineurin (CAN))

Bindungsspezifität: AA 349-505

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CAN Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Marke: BD Pharmingen™

Kreuzreaktivität: Human

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human beta1 isoform of calcineurin A Subunit aa. 349-505

Klon: G182-1847

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Applications include immunoprecipitation (1-2 µg/ml) and western blot analysis (1-2 µg/ml). Jurkat human T cells (ATCC TIB-152)2, HeLa human carcinoma cells (ATCC CCL-2), and A-431 human epidermal carcinoma cells (ATCC CRL-1555) are suggested as positive controls. A431 cell lysate (ABIN968533) and HeLa cell lysate (ABIN968535) are also available as ready-to-use positive western blot control.

Kommentare:

Related Products: ABIN967389, ABIN968533, ABIN968535

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-CAN Antikörper (ABIN967510)

Lai, Burnett, Wolosker, Blackshaw, Snyder: "Cain, a novel physiologic protein inhibitor of calcineurin." in: The Journal of biological chemistry, Vol. 273, Issue 29, pp. 18325-31, (1998) (PubMed).

Husi, Luyten, Zurini: "Mapping of the immunophilin-immunosuppressant site of interaction on calcineurin." in: The Journal of biological chemistry, Vol. 269, Issue 19, pp. 14199-204, (1994) (PubMed).

Fruman, Klee, Bierer, Burakoff: "Calcineurin phosphatase activity in T lymphocytes is inhibited by FK 506 and cyclosporin A." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 9, pp. 3686-90, (1992) (PubMed).

Details zu CAN

Target: CAN (Calcineurin (CAN))

Andere Bezeichnung: Calcineurin (CAN Produkte)

Hintergrund: Calcineurin is a Ca2+- and calmodulin-dependent serine/threonine phosphatase which consists of two subunits. The A subunit (~61 kDa) is catalytic and has calmodulinbinding activity, the B subunit (~19 kDa), which is myristylated at its N-terminus, has Ca[2+]-binding activity. Calcineurin is expressed ubiquitously in eukaryotic cells, including yeast. The protein is highly expressed in mammalian brain, and has been implicated in a number of calcium-dependent pathways in the nervous system, including regulation of neurotransmitter release. Calcineurin also plays a key role in T cell receptor-mediated signal transduction pathways. Calcineurin dephosphorylates components of NF-AT (Nuclear Factor of Activated T cells), allowing NF-AT to translocate to the nucleus where it participates with AP-1 in activation of multiple cytokine and surface receptor genes. Inhibition of calcineurin by immunosuppressive agents such as cyclosporin A and FK506 correlates with the suppression of early T cell activation events. These immunosuppressive agents bind to specific intracellular proteins, called immunophilins, to form complexes that inhibit calcineurin’s phosphatase activity. In the presence of these complexes, NF-AT remains phosphorylated and thus cannot translocate to the nucleus. A novel protein, Cain (calcineurin inhibitor), has been described which displays a similar pattern of neuronal and brain expression and has been shown to bind and inhibit calcineurin activity in vivo.
G182-1847 recognizes the alpha and beta isoforms of the A subunit of calcineurin (~61 kDa). It reacts with an epitope in the autoinhibitory domain. It does not recognize the B subunit of calcineurin. A recombinant tag-fusion protein containing amino acids 349-505 of the human beta1 isoform of calcineurin A subunit was used as immunogen.

Molekulargewicht: 61 kDa