Topoisomerase I Antikörper

Produktnummer ABIN967555

Produktdetails anti-Topoisomerase I Antikörper

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Topoisomerase I Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Marke: BD Pharmingen™

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Klon: C-21

Isotyp: IgM

Anwendungsinformationen

Applikationshinweise: The C-21 antibody is recommended for western blot analysis (1-2 µg/ml). A-431 human epidermal carcinoma cells (ATCC CRL-1555) or K562 human leukemia cells (ATCC CCL-243) may be used as a positive control for this application.

Kommentare:

Related Products: ABIN967389, ABIN968533

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-Topoisomerase I Antikörper (ABIN967555)

Tazi, Rossi, Labourier, Gallouzi, Brunel, Antoine: "DNA topoisomerase I: customs officer at the border between DNA and RNA worlds?" in: Journal of molecular medicine (Berlin, Germany), Vol. 75, Issue 11-12, pp. 786-800, (1998) (PubMed).

Sugimoto, Tsukahara, Oh-hara, Isoe, Tsuruo: "Decreased expression of DNA topoisomerase I in camptothecin-resistant tumor cell lines as determined by a monoclonal antibody." in: Cancer research, Vol. 50, Issue 21, pp. 6925-30, (1990) (PubMed).

Details zu Topoisomerase I

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Andere Bezeichnung: DNA Topoisomerase I (TOP1 Produkte)

Synonyme: CG6146 antikoerper, Dmel\\CG6146 antikoerper, TOP1 antikoerper, TopI antikoerper, Topo I antikoerper, TopoI antikoerper, dTopoI antikoerper, l(1)G0134 antikoerper, l(1)G0201 antikoerper, l(1)G0229 antikoerper, l(1)G0278 antikoerper, l(1)top1 antikoerper, top1 antikoerper, topo I antikoerper, topoI antikoerper, DNA topoisomerase 1 beta antikoerper, DNA topoisomerase I alpha antikoerper, MGO1 antikoerper, MGOUN 1 antikoerper, MTE17.1 antikoerper, MTE17_1 antikoerper, TOP1BETA antikoerper, TOPOISOMERASE 1 antikoerper, TOPI antikoerper, AI467334 antikoerper, D130064I21Rik antikoerper, Top-1 antikoerper, Ab2-086 antikoerper, topi antikoerper, Topoisomerase 1 antikoerper, DNA topoisomerase I antikoerper, DNA topoisomerase I alpha antikoerper, DNA topoisomerase 1 antikoerper, topoisomerase (DNA) I antikoerper, DNA topoisomerase I, gene 1 L homeolog antikoerper, Top1 antikoerper, top1 antikoerper, topA antikoerper, ZMO1193 antikoerper, MRAD2831_RS63095 antikoerper, Lferr_0016 antikoerper, PC1_2015 antikoerper, Za10_0136 antikoerper, Gbro_0575 antikoerper, Dd586_2108 antikoerper, Kvar_3044 antikoerper, Alvin_2069 antikoerper, Dacet_0849 antikoerper, Mrub_1615 antikoerper, Arnit_2962 antikoerper, Ndas_4953 antikoerper, Trad_2377 antikoerper, Acear_1653 antikoerper, Mahau_0844 antikoerper, Mesop_4109 antikoerper, Thein_1586 antikoerper, Theth_0241 antikoerper, TOP1ALPHA antikoerper, top-1 antikoerper, TOP1 antikoerper, top1.1.L antikoerper

Substanzklasse: Viral Protein

Hintergrund: DNA damage may be caused by various environmental factors, including radiation, mutagenic chemicals and copy errors which occur during DNA replication. The correction of DNA damage, so called proofreading functions of the cell, is achieved by numerous excision-repair enzymes. Topoisomerases alter the helical structure of DNA by introducing the transient breaking and rejoining of DNA strands, allowing other excision-repair enzymes to correct DNA errors. Topoisomerase I (Topo I) is a ubiquitous, soluble enzyme whose expression is fairly constant throughout the cell cycle. The related enzyme, Topo II, is a primarily insoluble structural protein whose expression varies between cell types and during the cell cycle. In addition to its role in DNA mismatch repair, Topo I displays kinase activity, phosphorylating serine-arginine rich (SR) splicing factors, and perhaps regulating gene expression by changing the splicing pattern of structural genes. DNA Topo I migrates at a molecular weight of 100 kDa in SDS-PAGE. Clone C-21 recognizes human DNA Topoisomerase I. The antibody is routinely tested by western blot analysis of A-431 cell lysates.

Molekulargewicht: 100 kDa

Pathways: Caspase Kaskade in der Apoptose, Stem Cell Maintenance