Ezrin Antikörper (pThr567)

Produktnummer ABIN967590

Produktdetails anti-Ezrin Antikörper

Target: Ezrin (EZR)

Bindungsspezifität: pThr567

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Ezrin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Marke: BD Pharmingen™

Kreuzreaktivität (Details): Predicted: Mouse, Rat

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Ezrin Peptide

Klon: J37-954-281-307

Isotyp: IgG1 kappa

Anwendungsinformationen

Kommentare:

Related Products: ABIN967389, ABIN968533, ABIN968534, ABIN968057

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-Ezrin Antikörper (ABIN967590)

Gautreau, Louvard, Arpin: "Morphogenic effects of ezrin require a phosphorylation-induced transition from oligomers to monomers at the plasma membrane." in: The Journal of cell biology, Vol. 150, Issue 1, pp. 193-203, (2000) (PubMed).

Tran Quang, Gautreau, Arpin, Treisman: "Ezrin function is required for ROCK-mediated fibroblast transformation by the Net and Dbl oncogenes." in: The EMBO journal, Vol. 19, Issue 17, pp. 4565-76, (2000) (PubMed).

Gautreau, Poullet, Louvard, Arpin: "Ezrin, a plasma membrane-microfilament linker, signals cell survival through the phosphatidylinositol 3-kinase/Akt pathway." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 13, pp. 7300-5, (1999) (PubMed).

Details zu Ezrin

Target: Ezrin (EZR)

Andere Bezeichnung: Ezrin (EZR Produkte)

Hintergrund: Ezrin is a member of the ERM (Ezrin-Radixin-Moesin) family of proteins that can function as a crosslinker between the actin cytoskeleton and the plasma membrane of the cell. Phosphorylation of threonine residues (Thr567 on Ezrin, Thr564 on Radixin and Thr558 on Moesin) has been reported to occur with stimulation of growth factors and is important for cytoskeletal rearrangements. Phosphorylation of Thr567 at the C-terminal F actin-binding domain has been reported to activate the conversion of Ezrin from a dormant soluble form in the cytosol to a membrane- and actin-binding conformation.
In addition to its reactivity to the human species, the peptide used to generate this antibody is either identical or has homology to analogous regions of mouse and rat Ezrin, Radixin and Moesin. The J37-954.281.307 monoclonal antibody has been reported to recognize phosphorylated T567 on active Ezrin in addition to crossreacting on phosphorylated T564 on Radixin and phosphorylated T558 on Moesin.

Molekulargewicht: 75-80 kDa

Pathways: Maintenance of Protein Location