Retinoblastoma 1 Antikörper (pSer807)
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- Target Alle Retinoblastoma 1 (RB1) Antikörper anzeigen
- Retinoblastoma 1 (RB1)
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Bindungsspezifität
- pSer807
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Reaktivität
- Human
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser Retinoblastoma 1 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB)
- Marke
- BD Pharmingen™
- Produktmerkmale
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Please refer to us for technical protocols. - Aufreinigung
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Immunogen
- Phosphorylated Human Rb Peptide
- Klon
- J112-906
- Isotyp
- IgG2a kappa
- Top Product
- Discover our top product RB1 Primärantikörper
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- Kommentare
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Related Products: ABIN967389
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 0.5 mg/mL
- Buffer
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C
- Informationen zur Lagerung
- Store undiluted at 4°C.
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Pocket proteins and cell cycle control." in: Oncogene, Vol. 24, Issue 17, pp. 2796-809, (2005) (PubMed).
: "Cyclin C/cdk3 promotes Rb-dependent G0 exit." in: Cell, Vol. 117, Issue 2, pp. 239-51, (2004) (PubMed).
: "Differential regulation of retinoblastoma protein function by specific Cdk phosphorylation sites." in: The Journal of biological chemistry, Vol. 271, Issue 14, pp. 8313-20, (1996) (PubMed).
: "
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Pocket proteins and cell cycle control." in: Oncogene, Vol. 24, Issue 17, pp. 2796-809, (2005) (PubMed).
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- Target
- Retinoblastoma 1 (RB1)
- Andere Bezeichnung
- Rb (RB1 Produkte)
- Synonyme
- OSRC antikoerper, RB antikoerper, p105-Rb antikoerper, pRb antikoerper, pp110 antikoerper, Rb antikoerper, Rb-1 antikoerper, P105-RB antikoerper, PP110 antikoerper, RB11 antikoerper, zgc:154147 antikoerper, RB transcriptional corepressor 1 antikoerper, RB transcriptional corepressor like 1 antikoerper, retinoblastoma 1 antikoerper, RB1 antikoerper, RBL1 antikoerper, Rb1 antikoerper, rb1 antikoerper
- Hintergrund
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The retinoblastoma gene product (Rb) is well known as a tumor suppressor and is either absent or mutated in many human tumors. Retrovirus-mediated gene transfer of the wild-type Rb gene into several Rb mutant neoplastic cell lines suppresses their tumorgenicity. Rb is a 110-kDa nuclear phosphoprotein that undergoes differential phosphorylation during the cell cycle. During G1 phase, Rb is predominantly in a hypophosphorylated state. It becomes increasingly phosphorylated throughout the cell cycle until late mitosis, when substantial dephosphorylation occurs. Hypophosphorylated Rb interacts with a number of cellular proteins including the E2F transcription factor, several cyclins, RBP-1, RBP-2, c-Abl, c-myc, N-myc, and p46. Phosphorylation of Rb at various sites, by Cyclin-dependent protein kinases, inhibits the binding of Rb to these proteins. Rb is thought to mediate its effects, in part, via the repression of genes required for proliferation. For example, Rb is specifically recruited to promoters containing E2F sites and actively represses E2F mediated transcription. Rb also stimulates the activity of other transcription factors, although the mechanisms are less clearly defined. Thus, Rb appears to regulate transcription in its aim to control cell growth.
The J112-906 monoclonal antibody recognizes Rb phosphorylated at serines 807 and 811 (pS807/pS811), which regulate c-Abl binding and cell cycle progression. The orthologous phosphorylation sites in mouse and rat Rb are pS800/pS804 and pS778/pS782, respectively. - Molekulargewicht
- 110 kDa
- Pathways
- Zellzyklus, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagie
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