NFkB Antikörper (pSer529)

Produktnummer ABIN967610

Produktdetails anti-NFkB Antikörper

Target: NFkB (Nuclear Factor kappa B (NFkB))

Bindungsspezifität: pSer529

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NFkB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Intracellular Staining (ICS)

Marke: BD Pharmingen™

Produktmerkmale: 1. Please refer to us for technical protocols.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human NF-kappaB p65 Peptide

Klon: K10-895-12-50

Isotyp: IgG2b kappa

Anwendungsinformationen

Kommentare:

Related Products: ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-NFkB Antikörper (ABIN967610)

Natoli, Saccani, Bosisio, Marazzi: "Interactions of NF-kappaB with chromatin: the art of being at the right place at the right time." in: Nature immunology, Vol. 6, Issue 5, pp. 439-45, (2005) (PubMed).

Siebenlist, Brown, Claudio: "Control of lymphocyte development by nuclear factor-kappaB." in: Nature reviews. Immunology, Vol. 5, Issue 6, pp. 435-45, (2005) (PubMed).

Viatour, Merville, Bours, Chariot: "Phosphorylation of NF-kappaB and IkappaB proteins: implications in cancer and inflammation." in: Trends in biochemical sciences, Vol. 30, Issue 1, pp. 43-52, (2005) (PubMed).

Details zu NFkB

Target: NFkB (Nuclear Factor kappa B (NFkB))

Andere Bezeichnung: NF-kappa B (NFkB Produkte)

Synonyme: EBP-1 antikoerper, KBF1 antikoerper, NF-kB1 antikoerper, NF-kappa-B antikoerper, NF-kappaB antikoerper, NFKB-p105 antikoerper, NFKB-p50 antikoerper, NFkappaB antikoerper, p105 antikoerper, p50 antikoerper, NF-kB antikoerper, NF-KB1 antikoerper, NF-kappaB1 antikoerper, p50/p105 antikoerper, nuclear factor kappa B subunit 1 antikoerper, nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 antikoerper, NFKB1 antikoerper, Nfkb1 antikoerper

Hintergrund: Nuclear factor kB (NF-kB) is a ubiquitously expressed transcription factor that regulates the expression of 200-300 genes. It is crucial for basic cellular responses to stress and pathogens, such as proliferation, survival, development, and apoptosis. The most studied NF-kB complex consists of the p50 (also known as NF-kB1) and p65 (also known as REL-A) subunits, both containing a 300-amino acid region with homology to the Rel proto-oncogene product (RH domain). The RH domain contains motifs for dimerization, nuclear localization, and binding to specific DNA sequences. In addition to the RH domain, the p65 subunit contains the transactivation domain, which is responsible for the interaction with the inhibitor IkB and which contains phosphorylation sites. In most cell types, the p50/p65 heterodimer is located within the cytoplasm complexed to IkB. This complex prevents nuclear translocation and activity of NF-kB. In response to stimuli such as cytokines, LPS, DNA damage, and viral infections, IkB is phosphorylated at critical residues. This phosphorylation induces dissociation of the IkB/NF-kB complex, allowing the free heterodimeric NF-kB to translocate to the nucleus. Furthermore, optimal activation of NF-kB requires phosphorylation in the transactivation domain of p65. In the nucleus, activated NF-kB dimers bind to the kB sites within promoters and enhancers and function as transcriptional activators. The K10-895.12.50 monoclonal antibody recognizes the phosphorylated serine 529 (pS529) in the transactivation domain of human NF-kB p65 subunit.

Molekulargewicht: 65 kDa

Pathways: Ubiquitin Proteasome Pathway, S100 Proteine