TRAF2 Antikörper (AA 93-199)

Produktnummer ABIN967631

Produktdetails anti-TRAF2 Antikörper

Target: TRAF2 (TNF Receptor-Associated Factor 2 (TRAF2))

Bindungsspezifität: AA 93-199

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TRAF2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Marke: BD Pharmingen™

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human TRAF2 aa. 93-199

Klon: C90-481

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Applications include western blot analysis (1-2 µg/ml). Human cell lines including Jurkat T cells (ATCC TIB-152), 293 adenovirus-transformed kidney cells (ATCC CRL-1573) and Daudi Burkitt lymphoma cells (ATCC CCL-213) are recommended as positive controls.

Kommentare:

Related Products: ABIN967389, ABIN968537

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-TRAF2 Antikörper (ABIN967631)

Arch, Gedrich, Thompson: "Translocation of TRAF proteins regulates apoptotic threshold of cells." in: Biochemical and biophysical research communications, Vol. 272, Issue 3, pp. 936-45, (2000) (PubMed).

Arch, Gedrich, Thompson: "Tumor necrosis factor receptor-associated factors (TRAFs)--a family of adapter proteins that regulates life and death." in: Genes & development, Vol. 12, Issue 18, pp. 2821-30, (1998) (PubMed).

Shu, Takeuchi, Goeddel: "The tumor necrosis factor receptor 2 signal transducers TRAF2 and c-IAP1 are components of the tumor necrosis factor receptor 1 signaling complex." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 24, pp. 13973-8, (1997) (PubMed).

Tsitsikov, Wright, Geha: "CD30 induction of human immunodeficiency virus gene transcription is mediated by TRAF2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 4, pp. 1390-5, (1997) (PubMed).

Yeh, Shahinian, Speiser, Kraunus, Billia, Wakeham, de la Pompa, Ferrick, Hum, Iscove, Ohashi, Rothe, Goeddel, Mak: "Early lethality, functional NF-kappaB activation, and increased sensitivity to TNF-induced cell death in TRAF2-deficient mice." in: Immunity, Vol. 7, Issue 5, pp. 715-25, (1997) (PubMed).

Hsu, Shu, Pan, Goeddel: "TRADD-TRAF2 and TRADD-FADD interactions define two distinct TNF receptor 1 signal transduction pathways." in: Cell, Vol. 84, Issue 2, pp. 299-308, (1996) (PubMed).

Rothe, Sarma, Dixit, Goeddel: "TRAF2-mediated activation of NF-kappa B by TNF receptor 2 and CD40." in: Science (New York, N.Y.), Vol. 269, Issue 5229, pp. 1424-7, (1995) (PubMed).

Details zu TRAF2

Target: TRAF2 (TNF Receptor-Associated Factor 2 (TRAF2))

Andere Bezeichnung: TRAF2 (TRAF2 Produkte)

Synonyme: MGC:45012 antikoerper, TRAP antikoerper, TRAP3 antikoerper, TANK antikoerper, AI325259 antikoerper, TNF-receptor-associated factor 2 antikoerper, conjugal transfer protein TraF antikoerper, TNF receptor associated factor 2 antikoerper, TNF receptor-associated factor 2 antikoerper, Tnf receptor-associated factor 2 antikoerper, LOC661716 antikoerper, traF2 antikoerper, TRAF2 antikoerper, Traf2 antikoerper

Hintergrund: TRAFs (TNF receptor associated factors) are signal transducing molecules which participate in forming TNF/Nerve Growth Factor receptor-associated signalling complexes. TRAF proteins share a novel C-terminal homology region, the TRAF” domain, a coiled-coiled region and the majority have Zinc/RING finger motifs. At least six TRAF proteins (TRAFs 1-6) have been identified. These proteins are cytoplasmic adapters, they bind to cytoplasmic domains of various receptors and can function to recruit other proteins to a signaling complex thereby promoting intracellular signal transduction. Specifically, TRAF2 has been shown to interact with the following surface receptors: TNFRII, CD27, CD30, CD40, 4-1BB, Ox40, HVEM/ATAR and LMP-1. TRAF2 has also been shown to associate with intracellular proteins, including TRADD, FADD, I-TRAF/TANK, TRIP, A20, c-IAP1 and 2, Casper, RIP and NIK. Thus, the interaction of TRAF2 with these various receptors and intracellular proteins plays an important role modulating downstream events which can result in inducing cell death or keeping cells alive. For example, cells deficient in TRAF2 demonstrated increased sensitivity to TNF-induced cell death, but a decrease in TNF-induced JNK activation, and little change on NF-kB activation. TRAF2 is detected at ~ 53 kDa in western blot analysis. The C90-481 antibody reacts with human TRAF2. A synthetic protein freagment corresponding to amino acids 93-199 of human TRAF2 was used as immunogen.

Molekulargewicht: 53 kDa

Pathways: NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Unfolded Protein Response, S100 Proteine