ANK2 Antikörper

Produktnummer ABIN967633

Produktdetails anti-ANK2 Antikörper

Target: ANK2 (Ankyrin 2, Neuronal (ANK2))

Reaktivität: Human, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ANK2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)

Marke: BD Pharmingen™

Kreuzreaktivität: Ratte (Rattus)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Ankyrin B Spectrin Binding Domain Peptide

Klon: 2-20

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Neonatal rat brain membranes are suggested for use as the positive control.

Kommentare:

Related Products: ABIN967389, ABIN968545, ABIN968546

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4° C.

Referenzen für anti-ANK2 Antikörper (ABIN967633)

Scotland, Zhou, Benveniste, Bennett: "Nervous system defects of AnkyrinB (-/-) mice suggest functional overlap between the cell adhesion molecule L1 and 440-kD AnkyrinB in premyelinated axons." in: The Journal of cell biology, Vol. 143, Issue 5, pp. 1305-15, (1999) (PubMed).

Kordeli, Lambert, Bennett: "AnkyrinG. A new ankyrin gene with neural-specific isoforms localized at the axonal initial segment and node of Ranvier." in: The Journal of biological chemistry, Vol. 270, Issue 5, pp. 2352-9, (1995) (PubMed).

Bennett, Gilligan: "The spectrin-based membrane skeleton and micron-scale organization of the plasma membrane." in: Annual review of cell biology, Vol. 9, pp. 27-66, (1994) (PubMed).

Otto, Kunimoto, McLaughlin, Bennett: "Isolation and characterization of cDNAs encoding human brain ankyrins reveal a family of alternatively spliced genes." in: The Journal of cell biology, Vol. 114, Issue 2, pp. 241-53, (1991) (PubMed).

Kordeli, Davis, Trapp, Bennett: "An isoform of ankyrin is localized at nodes of Ranvier in myelinated axons of central and peripheral nerves." in: The Journal of cell biology, Vol. 110, Issue 4, pp. 1341-52, (1990) (PubMed).

Details zu ANK2

Target: ANK2 (Ankyrin 2, Neuronal (ANK2))

Andere Bezeichnung: Ankyrin B (ANK2 Produkte)

Synonyme: ANK-2 antikoerper, LQT4 antikoerper, brank-2 antikoerper, AI835472 antikoerper, AW491075 antikoerper, Ank-2 antikoerper, Ank3 antikoerper, CHANK2 antikoerper, ANK2 antikoerper, ank2 antikoerper, zgc:101738 antikoerper, ANKB antikoerper, ankyrin 2 antikoerper, ankyrin 2, brain antikoerper, ankyrin-2 antikoerper, ankyrin 2b, neuronal antikoerper, ANK2 antikoerper, Ank2 antikoerper, LOC539238 antikoerper, ank2b antikoerper, LOC100465763 antikoerper

Hintergrund: Ankyrins are a family of proteins which were first discovered in erythrocytes and shown to bind to spectrin, a primary component of the cytoskeletal architecture. They have since been shown to be expressed at very high levels in the vertebrate brain and are also found in other cell types. Ankyrins function by acting as adaptors to link integral membrane proteins, including cell adhesion and ion channel molecules to the cytoskeleton. The structure of ankyrin consists of three domains, two highly conserved N-terminal and one C-terminal variable domain. The conserved regions contain a membrane-binding domain of ~89-95 kDa and a spectrin-binding domain of ~62 kDa, while the variable region has pre-mRNA alternative splice sites. Two ankyrins have been extensively characterized: ankryin R is the product of the ANK1 gene and is primarily expressed in the brain, although alternatively spliced forms are found in erythrocytes, ankyrin B is the product of the ANK2 gene, located on a different chromosome from ANK1, and is primarly expressed in neonatal and adult brain but is also found in heart, lymphocytes and platelets. Studies done on ankyrinB (-/-) knockout mice, which die by postnatal day 21, have demonstrated a link between the L1 cell adhesion molecule and ankyrin B in premyelinated axons. Isoforms of ankyrin B have been reported to be detectable ranging from approximately 440, 220, 150 and 110 kDa in western blot analysis, depending on the tissue-type of origin. This antibody is routinely tested by western blot analysis.

Molekulargewicht: 110-440 kDa

Pathways: Cell-Cell Junction Organization