IFNGR1 Antikörper

Produktnummer ABIN967652

Produktdetails anti-IFNGR1 Antikörper

Target: IFNGR1 (Interferon gamma Receptor 1 (IFNGR1))

Reaktivität: Maus

Wirt: Armenischer Hamster

Klonalität: Monoklonal

Konjugat: Dieser IFNGR1 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP)

Marke: BD Pharmingen™

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Purified preparation of soluble recombinant mouse IFN-gammaRalpha chain protein

Klon: 2E2

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Immunofluorescent Staining and Flow Cytometric Analysis: The purified form of 2E2 can be used for the immunofluorescent staining (Less or equal than 1 µg antibody/10e6 cells) and flow cytometric analysis of normal mouse cells or cell lines to measure their expressed levels of IFN-gammaRalpha. An appropriate purified immunoglobulin isotype control is A19-3. A three-layer staining protocol is recommended for maximizing the detection IFN-gammaRalpha chains expressed by cells as detailed in the figure legend.
2E2 is a nonblocking antibody that can be used for the unobstructed immunofluorescent staining and flow cytometric analysis of cells in systems where the ligand (i.e., IFN-gamma) for IFN-gamma receptors is present. Immunoprecipitation: The 2E2 antibody has been reported to be useful for the immunoprecipitation of IFN-gammaRalpha chains from lysates of cloned mouse T cells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4° C.

Referenzen für anti-IFNGR1 Antikörper (ABIN967652)

Bach, Szabo, Dighe, Ashkenazi, Aguet, Murphy, Schreiber: "Ligand-induced autoregulation of IFN-gamma receptor beta chain expression in T helper cell subsets." in: Science (New York, N.Y.), Vol. 270, Issue 5239, pp. 1215-8, (1996) (PubMed).

Details zu IFNGR1

Target: IFNGR1 (Interferon gamma Receptor 1 (IFNGR1))

Andere Bezeichnung: CD119 (IFNGR1 Produkte)

Synonyme: CD119 antikoerper, IFNGR antikoerper, IFN-gammaR antikoerper, Ifgr antikoerper, Ifngr antikoerper, Nktar antikoerper, IFNGR1 antikoerper, MGC129098 antikoerper, ifngr1 antikoerper, MGC194858 antikoerper, MGC194873 antikoerper, zgc:194858 antikoerper, cd119 antikoerper, ifngr antikoerper, DKFZp469K1232 antikoerper, interferon gamma receptor 1 antikoerper, cytokine receptor family member B17 antikoerper, IFNGR1 antikoerper, Ifngr1 antikoerper, crfb17 antikoerper, ifngr1 antikoerper

Hintergrund: The 2E2 antibody recognizes the extracellular region of the 90 kDa alpha chain subunit of the mouse interferon-gamma receptor (IFN-gammaRalpha, aka, CD119). The functionally active-form of the mouse IFN-gamma receptor consists of two (or more) subunits, with IFN-gammaRalpha responsible for IFN-gamma binding and both the IFN-gammaRalpha and IFN-gammaRbeta chains required for the transduction of biologic responses. IFN-gammaRalpha is expressed by a variety of cell lines and normal mouse cells (except mature erythrocytes) including T cells, B cells, NK cells, monocytes, neutrophils, fibroblasts, epithelial and endothelial cells. The 2E2 antibody is a non-neutralizing antibody, it does not block the binding of IFN-gamma to its receptor. The immunogen used to generate this hybridoma was a purified preparation of soluble recombinant mouse IFN-gammaRalpha chain protein.
Synonyms: IFN-gamma Receptor alpha chain

Pathways: Interferon-gamma Pathway