CSK Antikörper (AA 1-156)

Produktnummer ABIN967730

Produktdetails anti-CSK Antikörper

Target: CSK (C-Src tyrosine Kinase (CSK))

Bindungsspezifität: AA 1-156

Reaktivität: Human, Maus, Ratte, Hund, Huhn

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CSK Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Kreuzreaktivität: Maus, Human, Hund, Huhn

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat Csk aa. 1-156

Klon: 52-Csk

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968555, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-CSK Antikörper (ABIN967730)

Bénistant, Bourgaux, Chapuis, Mottet, Roche, Bali: "The COOH-terminal Src kinase Csk is a tumor antigen in human carcinoma." in: Cancer research, Vol. 61, Issue 4, pp. 1415-20, (2001) (PubMed).

Takayama, Tanaka, Nagai, Okada et al.: "Adenovirus-mediated overexpression of C-terminal Src kinase (Csk) in type I astrocytes interferes with cell spreading and attachment to fibronectin. Correlation with tyrosine phosphorylations of ..." in: The Journal of biological chemistry, Vol. 274, Issue 4, pp. 2291-7, (1999) (PubMed).

Wan, Bence, Hata, Kurosaki, Veillette, Huang: "Genetic evidence for a tyrosine kinase cascade preceding the mitogen-activated protein kinase cascade in vertebrate G protein signaling." in: The Journal of biological chemistry, Vol. 272, Issue 27, pp. 17209-15, (1997) (PubMed).

Bergman, Mustelin, Oetken, Partanen, Flint, Amrein, Autero, Burn, Alitalo: "The human p50csk tyrosine kinase phosphorylates p56lck at Tyr-505 and down regulates its catalytic activity." in: The EMBO journal, Vol. 11, Issue 8, pp. 2919-24, (1992) (PubMed).

Okada, Nada, Yamanashi, Yamamoto, Nakagawa: "CSK: a protein-tyrosine kinase involved in regulation of src family kinases." in: The Journal of biological chemistry, Vol. 266, Issue 36, pp. 24249-52, (1992) (PubMed).

Details zu CSK

Target: CSK (C-Src tyrosine Kinase (CSK))

Andere Bezeichnung: Csk (CSK Produkte)

Synonyme: AW212630 antikoerper, C-terminal Src kinase antikoerper, c-src tyrosine kinase antikoerper, CSK, non-receptor tyrosine kinase antikoerper, CSK antikoerper, Csk antikoerper

Hintergrund: The csk gene encodes a protein kinase that phosphorylates several Src family kinases including p60[c-src], p59[fyn], p53/56[lyn], p56[lck], and p62[yes]. This phosphorylation occurs specifically at the carboxy-terminal tyrosine, Y527. Csk (c-src kinase) was identified based on its ability to phosphorylate exogenous Src in mammalian src-deficient cells. Establishment of a mouse csk- cell line revealed that phosphorylation of Y527 in Src was reduced 20-50%. This correlated with an increase of Src kinase activity, thus indicating Csk is a negative regulator of src. Csk-deficient cells also show an elevation in the kinase activity of p59[fyn] and p53/56[lyn]. These data suggest that Csk may be a universal negative regulator of Src family kinases in vivo.

Molekulargewicht: 50 kDa

Pathways: T-Zell Rezeptor Signalweg, EGFR Signaling Pathway, Cell-Cell Junction Organization, CXCR4-mediated Signaling Events