rala Antikörper (AA 35-206)

Produktnummer ABIN967836

Produktdetails anti-rala Antikörper

Target: rala (Ras-related Protein Ral-A (rala))

Bindungsspezifität: AA 35-206

Reaktivität: Ratte, Hund, Huhn

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser rala Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Kreuzreaktivität: Ratte (Rattus), Huhn, Hund, Maus

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Please refer to us for technical protocols.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Ral A aa. 35-206

Klon: 8-Ral A

Isotyp: IgG2a kappa

Anwendungsinformationen

Kommentare:

Related Products: ABIN968545, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Referenzen für anti-rala Antikörper (ABIN967836)

Xu, Frankel, Jackson, Rotunda, Boshans, DSouza-Schorey, Foster: "Elevated phospholipase D activity in H-Ras- but not K-Ras-transformed cells by the synergistic action of RalA and ARF6." in: Molecular and cellular biology, Vol. 23, Issue 2, pp. 645-54, (2003) (PubMed).

Kinashi, Katagiri, Watanabe, Vanhaesebroeck, Downward, Takatsu: "Distinct mechanisms of alpha 5beta 1 integrin activation by Ha-Ras and R-Ras." in: The Journal of biological chemistry, Vol. 275, Issue 29, pp. 22590-6, (2000) (PubMed).

Goi, Rusanescu, Urano, Feig: "Ral-specific guanine nucleotide exchange factor activity opposes other Ras effectors in PC12 cells by inhibiting neurite outgrowth." in: Molecular and cellular biology, Vol. 19, Issue 3, pp. 1731-41, (1999) (PubMed).

Polakis, Weber, Nevins, Didsbury, Evans, Snyderman: "Identification of the ral and rac1 gene products, low molecular mass GTP-binding proteins from human platelets." in: The Journal of biological chemistry, Vol. 264, Issue 28, pp. 16383-9, (1989) (PubMed).

Olofsson, Chardin, Touchot, Zahraoui, Tavitian: "Expression of the ras-related ralA, rho12 and rab genes in adult mouse tissues." in: Oncogene, Vol. 3, Issue 2, pp. 231-4, (1988) (PubMed).

Details zu rala

Target: rala (Ras-related Protein Ral-A (rala))

Andere Bezeichnung: Ral A (rala Produkte)

Synonyme: 3010001O15Rik antikoerper, AW322615 antikoerper, Ral antikoerper, Rasl1 antikoerper, RAL antikoerper, v-ral simian leukemia viral oncogene A (ras related) antikoerper, RAS like proto-oncogene A antikoerper, Rala antikoerper, RALA antikoerper

Hintergrund: Ral is a low molecular weight GTP-binding protein belonging to the Ras superfamily of GTP-binding proteins and shows 50% amino acid identity to Ras. Ral cDNA clones have been isolated from human placenta, human pheochromocytoma, simian ß-lymphocyte, and marine ray electric lobe cDNA libraries. In humans, cDNA sequences for Ral A and RalB have been determined. The predicted amino acid sequences show 85% identity. Both proteins consist of 206 amino acids with a predicted molecular weight of 24kDa. However, Ral A isolated from human platelets shows an apparent molecular weight of 26-28kDa. Ral A mRNA levels in adult mouse tissues appear to be highest in testes, ovary, and brain, with lower levels found in the liver, spleen, kidney, thymus, heart, and salivary glands. Similar results have been observed in rat tissues. This antibody is routinely tested by western blot analysis.

Molekulargewicht: 24-28 kDa

Pathways: Neurotrophin Signalübertragung, SARS-CoV-2 Protein Interaktom