ZAP70 Antikörper (AA 468-619)

Produktnummer ABIN967849

Produktdetails anti-ZAP70 Antikörper

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Bindungsspezifität: AA 468-619

Reaktivität: Human, Maus, Ratte, Huhn

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ZAP70 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Kreuzreaktivität: Maus, Ratte (Rattus), Huhn

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human ZAP-70 Kinase aa. 468-619

Klon: 29-ZAP70 Kinase

Isotyp: IgG2a

Anwendungsinformationen

Kommentare:

Related Products: ABIN968537, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Referenzen für anti-ZAP70 Antikörper (ABIN967849)

Michel, Attal-Bonnefoy, Mangino, Mise-Omata, Acuto: "CD28 as a molecular amplifier extending TCR ligation and signaling capabilities." in: Immunity, Vol. 15, Issue 6, pp. 935-45, (2001) (PubMed).

Mora, Stanley, Armistead, Chan, Boothby: "Inefficient ZAP-70 phosphorylation and decreased thymic selection in vivo result from inhibition of NF-kappaB/Rel." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 10, pp. 5628-35, (2001) (PubMed).

Maccalli, Pisarra, Vegetti, Sensi, Parmiani, Anichini: "Differential loss of T cell signaling molecules in metastatic melanoma patients' T lymphocyte subsets expressing distinct TCR variable regions." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 12, pp. 6912-23, (2000) (PubMed).

Arpaia, Shahar, Dadi, Cohen, Roifman: "Defective T cell receptor signaling and CD8+ thymic selection in humans lacking zap-70 kinase." in: Cell, Vol. 76, Issue 5, pp. 947-58, (1994) (PubMed).

Chan, Kadlecek, Elder, Filipovich, Kuo, Iwashima, Parslow, Weiss: "ZAP-70 deficiency in an autosomal recessive form of severe combined immunodeficiency." in: Science (New York, N.Y.), Vol. 264, Issue 5165, pp. 1599-601, (1994) (PubMed).

Details zu ZAP70

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Andere Bezeichnung: ZAP-70 (ZAP70 Produkte)

Synonyme: SRK antikoerper, STCD antikoerper, STD antikoerper, TZK antikoerper, ZAP-70 antikoerper, 70kDa antikoerper, AI327364 antikoerper, Srk antikoerper, mrtle antikoerper, mur antikoerper, zap70 antikoerper, MGC80670 antikoerper, MGC88962 antikoerper, ZAP70 antikoerper, zgc:110383 antikoerper, zeta chain of T-cell receptor associated protein kinase 70 antikoerper, putative tyrosine-protein kinase zap-70 antikoerper, zeta-chain (TCR) associated protein kinase antikoerper, zeta chain of T cell receptor associated protein kinase 70 antikoerper, zeta-chain (TCR) associated protein kinase 70kDa antikoerper, zeta chain of T cell receptor associated protein kinase 70kDa L homeolog antikoerper, zeta chain of T cell receptor associated protein kinase 70kDa antikoerper, tyrosine-protein kinase ZAP-70 antikoerper, ZAP70 antikoerper, Smp_149470 antikoerper, Zap70 antikoerper, zap70.L antikoerper, zap70 antikoerper, LOC707555 antikoerper, LOC100342021 antikoerper, LOC100390922 antikoerper, LOC100542877 antikoerper

Hintergrund: ZAP-70 is a protein tyrosine kinase (PTK) that associates with the zeta subunit of the T cell antigen receptor (TCR) and undergoes tyrosine phosphorylation following TCR stimulation. ZAP-70 contains two SH2-like domains with the PTK domain being at the C-terminus. It appears that both ZAP-70 and Syk are recruited to the phosphorylated CD3 and zeta subunits after TCR stimulation. The src-family PTKs, lck, and fyn, seem to play a role upstream of ZAP-70 and Syk where they phosphorylate CD3 and zeta. Thus both Src- and ZAP-70/Syk-family PTKs are critical for efficient induction of tyrosine phosphoproteins in heterologous cells. The significance of ZAP-70 in mediating TCR signal transduction has been confirmed by showing that ZAP-70 activity is absent in an autosomal recessive form of severe combined immunodeficiency (SCID). This is due to mutations affecting the ZAP-70 kinase domain which affect the stability of the protein. The levels of lck and fyn appear to be normal in the same cells. Results of the ZAP-70 mutations in these patients include an unusual absence of peripheral CD8+ T cells and an abundance of CD4+ T cells in peripheral lymphoid organs. These results indicate that these two populations of T cells develop through utilization of different intracellular signaling pathways.

Molekulargewicht: 70 kDa

Pathways: T-Zell Rezeptor Signalweg, Ubiquitin Proteasome Pathway