CDKN1B Antikörper (AA 1-197)

Produktnummer ABIN967850

Produktdetails anti-CDKN1B Antikörper

Target: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Bindungsspezifität: AA 1-197

Reaktivität: Human, Maus, Ratte, Hund, Huhn, Frosch

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CDKN1B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Kreuzreaktivität: Human, Hund, Ratte (Rattus), Huhn, Frosch

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse Kip1 aa. 1-197

Klon: 57-Kip1-p27

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968535, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-CDKN1B Antikörper (ABIN967850)

Guo, Packard, Krolewski, Harris, Manglapus, Schwob: "Expression of pax6 and sox2 in adult olfactory epithelium." in: The Journal of comparative neurology, Vol. 518, Issue 21, pp. 4395-418, (2011) (PubMed).

Martín-Ibáñez, Crespo, Urbán, Sergent-Tanguy, Herranz, Jaumot, Valiente, Long, Pineda, Andreu, Rubenstein, Marín, Georgopoulos, Mengod, Fariñas, Bachs, Alberch, Canals: "Ikaros-1 couples cell cycle arrest of late striatal precursors with neurogenesis of enkephalinergic neurons." in: The Journal of comparative neurology, Vol. 518, Issue 3, pp. 329-51, (2010) (PubMed).

Poché, Furuta, Chaboissier, Schedl, Behringer: "Sox9 is expressed in mouse multipotent retinal progenitor cells and functions in Müller glial cell development." in: The Journal of comparative neurology, Vol. 510, Issue 3, pp. 237-50, (2008) (PubMed).

Barnouin, Dubuisson, Child, Fernandez de Mattos, Glassford, Medema, Mann, Lam: "H2O2 induces a transient multi-phase cell cycle arrest in mouse fibroblasts through modulating cyclin D and p21Cip1 expression." in: The Journal of biological chemistry, Vol. 277, Issue 16, pp. 13761-70, (2002) (PubMed).

Williamson, Dadmanesh, Koeffler: "BRCA1 transactivates the cyclin-dependent kinase inhibitor p27(Kip1)." in: Oncogene, Vol. 21, Issue 20, pp. 3199-206, (2002) (PubMed).

Frey, Clark, Leontieva, Uronis, Black, Black: "Protein kinase C signaling mediates a program of cell cycle withdrawal in the intestinal epithelium." in: The Journal of cell biology, Vol. 151, Issue 4, pp. 763-78, (2000) (PubMed).

Polyak, Kato, Solomon, Sherr, Massague, Roberts, Koff: "p27Kip1, a cyclin-Cdk inhibitor, links transforming growth factor-beta and contact inhibition to cell cycle arrest." in: Genes & development, Vol. 8, Issue 1, pp. 9-22, (1994) (PubMed).

Details zu CDKN1B

Target: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Andere Bezeichnung: p27 Kip1 (CDKN1B Produkte)

Synonyme: CDKN4 antikoerper, KIP1 antikoerper, MEN1B antikoerper, MEN4 antikoerper, P27KIP1 antikoerper, cdkn1b antikoerper, sb:cb611 antikoerper, wu:fb63g10 antikoerper, wu:fb64g10 antikoerper, wu:fe18e03 antikoerper, CDKN1B antikoerper, Kip1 antikoerper, p27 antikoerper, FAM14D antikoerper, ISG12 antikoerper, ISG12A antikoerper, P27 antikoerper, Cdki1b antikoerper, AA408329 antikoerper, AI843786 antikoerper, p27Kip1 antikoerper, cdkn1bl antikoerper, cyclin dependent kinase inhibitor 1B antikoerper, cyclin-dependent kinase inhibitor 1Bb antikoerper, interferon alpha inducible protein 27 antikoerper, cyclin-dependent kinase inhibitor 1B antikoerper, cyclin-dependent kinase inhibitor 1Ba antikoerper, CDKN1B antikoerper, cdkn1bb antikoerper, IFI27 antikoerper, Cdkn1b antikoerper, cdkn1ba antikoerper

Hintergrund: Kip1 is a cyclin-dependent kinase (cdk) inhibitor that was identified as a result of its role in TGFbeta-induced G1 phase arrest and cell-cell contact. In vitro , p27 [Kip1] binds tightly to Cyclin D-Cdk4, Cyclin E-Cdk2, and Cyclin A-Cdk2 complexes and inhibits their activity. In normal cells, Kip1 is sequestered and its activity gradually decreases as cells reach S phase. The addition of TGF-beta in early G1 blocks this decline in activity, by preventing the sequestration of Kip1. TGF-beta also reduces the levels of Cdk4. Kip1 preferentially binds Cyclin D-Cdk4, but the lower levels of Cdk4 in TGF-beta treated cells allow Kip1 to be available for binding to Cyclin E-Cdk2 and Cyclin A-Cdk2. Kip1 is structurally related to Cip1/WAF1, having a similar 60 amino acid sequence in the N-terminal region. A 52 amino acid Kip1 peptide (residues 28-79) from this region is sufficient to inhibit cdk activity in vitro. This antibody is routinely tested by western blot analysis.

Molekulargewicht: 27 kDa

Pathways: Zellzyklus, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagie