JUP Antikörper (AA 553-738)

Produktnummer ABIN967860

Produktdetails anti-JUP Antikörper

Target: JUP (Junction Plakoglobin (JUP))

Bindungsspezifität: AA 553-738

Reaktivität: Human, Maus, Ratte, Huhn, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser JUP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Kreuzreaktivität: Huhn, Hund, Maus, Ratte (Rattus)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human gamma-Catenin aa. 553-738

Klon: 15-gamma

Isotyp: IgG2a

Anwendungsinformationen

Kommentare:

Related Products: ABIN968535, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-JUP Antikörper (ABIN967860)

Akins, Greer: "Axon behavior in the olfactory nerve reflects the involvement of catenin-cadherin mediated adhesion." in: The Journal of comparative neurology, Vol. 499, Issue 6, pp. 979-89, (2007) (PubMed).

Mary, Charrasse, Meriane, Comunale, Travo, Blangy, Gauthier-Rouvière: "Biogenesis of N-cadherin-dependent cell-cell contacts in living fibroblasts is a microtubule-dependent kinesin-driven mechanism." in: Molecular biology of the cell, Vol. 13, Issue 1, pp. 285-301, (2002) (PubMed).

Merritt, Berika, Zhai, Kirk, Ji, Hardman, Garrod: "Suprabasal desmoglein 3 expression in the epidermis of transgenic mice results in hyperproliferation and abnormal differentiation." in: Molecular and cellular biology, Vol. 22, Issue 16, pp. 5846-58, (2002) (PubMed).

Peng, Mandai, Nakanishi, Ikeda, Asada, Momose, Shibamoto, Yanagihara, Shiozaki, Monden, Takeichi, Takai: "Restoration of E-cadherin-based cell-cell adhesion by overexpression of nectin in HSC-39 cells, a human signet ring cell gastric cancer cell line." in: Oncogene, Vol. 21, Issue 26, pp. 4108-19, (2002) (PubMed).

Müller, Choidas, Reichmann, Ullrich: "Phosphorylation and free pool of beta-catenin are regulated by tyrosine kinases and tyrosine phosphatases during epithelial cell migration." in: The Journal of biological chemistry, Vol. 274, Issue 15, pp. 10173-83, (1999) (PubMed).

McCrea, Turck, Gumbiner: "A homolog of the armadillo protein in Drosophila (plakoglobin) associated with E-cadherin." in: Science (New York, N.Y.), Vol. 254, Issue 5036, pp. 1359-61, (1992) (PubMed).

Details zu JUP

Target: JUP (Junction Plakoglobin (JUP))

Andere Bezeichnung: gamma-Catenin (JUP Produkte)

Synonyme: ARVD12 antikoerper, CTNNG antikoerper, DP3 antikoerper, DPIII antikoerper, PDGB antikoerper, PKGB antikoerper, arvd12 antikoerper, ctnng antikoerper, dp3 antikoerper, dpiii antikoerper, pdgb antikoerper, pkgb antikoerper, plak antikoerper, D930025P04Rik antikoerper, PG antikoerper, cb80 antikoerper, danPG antikoerper, fb11b06 antikoerper, jup antikoerper, wu:fb11b06 antikoerper, junction plakoglobin antikoerper, junction plakoglobin L homeolog antikoerper, Junction plakoglobin antikoerper, junction plakoglobin a antikoerper, junction plakoglobin S homeolog antikoerper, JUP antikoerper, Jup antikoerper, jup antikoerper, jup.L antikoerper, Tsp_06656 antikoerper, plak antikoerper, jupa antikoerper, jup.S antikoerper

Hintergrund: Gamma-Catenin (plakoglobin) was identified as a compenent of desmosomes where it associates with desmoglein. gamma-Catenin and beta-Catenin are closely related proteins that have significant homology with the Drosophila armadillo protein. In addition to complexing with E-Cadherin, gamma-Catenin and beta-Catenin have been observed in association with the intracellular domain of N-Cadherin. It has been proposed that one molecule of alpha-Catenin and at least one molecule of beta-Catenin and gamma-Catenin simultaneously bind to a single cadherin molecule. A 19 amino acid sequence of desmoglein (Dsg1) was found to be critical for binding of gamma-Catenin. This region has significant homology to the catenin-binding domain of classical cadherins, thus suggesting a common mechanism for gamma-Catenin localization at both adherens junctions and desmosomes.
This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

Molekulargewicht: 82 kDa

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location