BCAR1 Antikörper (AA 644-819)

Produktnummer ABIN967872

Produktdetails anti-BCAR1 Antikörper

Target: BCAR1 (Breast Cancer Anti-Estrogen Resistance 1 (BCAR1))

Bindungsspezifität: AA 644-819

Reaktivität: Human, Maus, Ratte, Hund, Huhn

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser BCAR1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Kreuzreaktivität: Human, Huhn, Hund, Maus

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat p130 [Cas] aa. 644-819

Klon: 21-p130[Cas]

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968536, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-BCAR1 Antikörper (ABIN967872)

Hermanto, Zong, Li, Wang: "RACK1, an insulin-like growth factor I (IGF-I) receptor-interacting protein, modulates IGF-I-dependent integrin signaling and promotes cell spreading and contact with extracellular matrix." in: Molecular and cellular biology, Vol. 22, Issue 7, pp. 2345-65, (2002) (PubMed).

Nagashima, Endo, Ogita, Kawana, Yamagishi, Kitabatake, Matsuda, Mochizuki: "Adaptor protein Crk is required for ephrin-B1-induced membrane ruffling and focal complex assembly of human aortic endothelial cells." in: Molecular biology of the cell, Vol. 13, Issue 12, pp. 4231-42, (2002) (PubMed).

Derkinderen, Toutant, Kadaré, Ledent, Parmentier, Girault: "Dual role of Fyn in the regulation of FAK+6,7 by cannabinoids in hippocampus." in: The Journal of biological chemistry, Vol. 276, Issue 41, pp. 38289-96, (2001) (PubMed).

Kook, Shim, Choi, Ahnn, Kim, Eom, Jung, Paik, Song: "Caspase-mediated cleavage of p130cas in etoposide-induced apoptotic Rat-1 cells." in: Molecular biology of the cell, Vol. 11, Issue 3, pp. 929-39, (2000) (PubMed).

Sakai, Iwamatsu, Hirano, Ogawa, Tanaka, Mano, Yazaki, Hirai: "A novel signaling molecule, p130, forms stable complexes in vivo with v-Crk and v-Src in a tyrosine phosphorylation-dependent manner." in: The EMBO journal, Vol. 13, Issue 16, pp. 3748-56, (1994) (PubMed).

Details zu BCAR1

Target: BCAR1 (Breast Cancer Anti-Estrogen Resistance 1 (BCAR1))

Andere Bezeichnung: p130 Cas (BCAR1 Produkte)

Hintergrund: P47 [v-crk] is the product of a transforming gene, v-crk, that was isolated from avian sarcoma viruses. The v-crk protein is a fusion product of the viral gag protein and a part of cellular crk that includes SH2 and SH3 domains. v-crk induced transformation increases tyrosine phosphorylation of several cellular proteins, including p130 [Cas]. p130 [Cas] is tightly associated with v-crk via the SH2 domain of v-crk. Tyrosine phosphorylation of p130 [Cas] occurs in conjunction with cellular transformation in cells that express v-src or v-crk. This phosphorylation leads to a change in p130 [Cas] localization from the cytoplasm to the cell membrane and, possibly, to the nucleus. Since p130 [Cas] also associates with v-src, it may be a v-src substrate. This antibody is routinely tested by western blot analysis.

Molekulargewicht: 130 kDa

Pathways: EGFR Signaling Pathway, Neurotrophin Signalübertragung, CXCR4-mediated Signaling Events, Platelet-derived growth Factor Receptor Signaling