DAP Kinase 1 Antikörper (AA 694-947)

Produktnummer ABIN967885

Produktdetails anti-DAP Kinase 1 Antikörper

Target: DAP Kinase 1 (DAPK1) (Death-Associated Protein Kinase 1 (DAPK1))

Bindungsspezifität: AA 694-947

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser DAP Kinase 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human DAP Kinase aa. 694-947

Klon: 17-DAP Kinase

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-DAP Kinase 1 Antikörper (ABIN967885)

Cohen, Feinstein, Kimchi: "DAP-kinase is a Ca2+/calmodulin-dependent, cytoskeletal-associated protein kinase, with cell death-inducing functions that depend on its catalytic activity." in: The EMBO journal, Vol. 16, Issue 5, pp. 998-1008, (1997) (PubMed).

Deiss, Feinstein, Berissi, Cohen, Kimchi: "Identification of a novel serine/threonine kinase and a novel 15-kD protein as potential mediators of the gamma interferon-induced cell death." in: Genes & development, Vol. 9, Issue 1, pp. 15-30, (1995) (PubMed).

Details zu DAP Kinase 1

Target: DAP Kinase 1 (DAPK1) (Death-Associated Protein Kinase 1 (DAPK1))

Andere Bezeichnung: DAP Kinase (DAPK1 Produkte)

Synonyme: DAPK1 antikoerper, MGC81366 antikoerper, si:ch211-66i11.1 antikoerper, wu:fj09c03 antikoerper, dapk antikoerper, MGC83745 antikoerper, 2310039H24Rik antikoerper, 2810425C21Rik antikoerper, AI642003 antikoerper, D13Ucla1 antikoerper, DAP-Kinase antikoerper, DAPK antikoerper, death-associated protein kinase 1 antikoerper, death associated protein kinase 1 L homeolog antikoerper, death associated protein kinase 1 antikoerper, death associated protein kinase 1 S homeolog antikoerper, LOC427465 antikoerper, dapk1.L antikoerper, DAPK1 antikoerper, dapk1 antikoerper, dapk1.S antikoerper, Dapk1 antikoerper

Hintergrund: Chronic exposure to extracellular signals such as interferons induce the inhibition of cell proliferation followed by cell death. The gene for DAP Kinase (Death Associated Protein Kinase) was identified using a novel approach named Technical Knockout. Briefly, Hela cells were transfected with an antisense cDNA expression library, then exposed to Interferon-gamma. The surviving cells, with their antisense cDNAs, were rescued and the protecting genes isolated. The DAP Kinase gene encodes a protein of 1423 amino acids, a molecular weight of 160kDa, a kinase domain at its amino terminal region, ankyrin repeats in the middle region, and a death domain at the extreme C-terminus. DAP Kinase phosphorylates at Ser/Thr residues in a Ca2+/Calmodulin-dependent fashion. It has been demonstrated that Ca2+/Calmodulin binds directly to DAP Kinase at its amino terminal region. In addition, immunostaining studies localized DAP Kinase in association with the actin filaments where it may phosphorylate myosin light chain. Thus, this novel cytoskeletal and Ca2+/Calmodulin-dependent protein kinase plays a role in interferon-gamma-induced cell death. This antibody is routinely tested by western blot analysis.

Molekulargewicht: 160 kDa

Pathways: MAPK Signalweg, Interferon-gamma Pathway