FADD Antikörper (AA 94-208)

Produktnummer ABIN967945

Produktdetails anti-FADD Antikörper

Target: FADD (Fas (TNFRSF6)-Associated Via Death Domain (FADD))

Bindungsspezifität: AA 94-208

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser FADD Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human FADD aa. 94-208

Klon: 1-FADD

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968533, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-FADD Antikörper (ABIN967945)

Chang, Xing, Pan, Algeciras-Schimnich, Barnhart, Yaish-Ohad, Peter, Yang: "c-FLIP(L) is a dual function regulator for caspase-8 activation and CD95-mediated apoptosis." in: The EMBO journal, Vol. 21, Issue 14, pp. 3704-14, (2002) (PubMed).

MacFarlane, Harper, Snowden, Dyer, Barnett, Pringle, Cohen: "Mechanisms of resistance to TRAIL-induced apoptosis in primary B cell chronic lymphocytic leukaemia." in: Oncogene, Vol. 21, Issue 44, pp. 6809-18, (2002) (PubMed).

Micheau, Thome, Schneider, Holler, Tschopp, Nicholson, Briand, Grütter: "The long form of FLIP is an activator of caspase-8 at the Fas death-inducing signaling complex." in: The Journal of biological chemistry, Vol. 277, Issue 47, pp. 45162-71, (2002) (PubMed).

Wieder, Essmann, Prokop, Schmelz, Schulze-Osthoff, Beyaert, Dörken, Daniel: "Activation of caspase-8 in drug-induced apoptosis of B-lymphoid cells is independent of CD95/Fas receptor-ligand interaction and occurs downstream of caspase-3." in: Blood, Vol. 97, Issue 5, pp. 1378-87, (2001) (PubMed).

Chinnaiyan, ORourke, Tewari, Dixit: "FADD, a novel death domain-containing protein, interacts with the death domain of Fas and initiates apoptosis." in: Cell, Vol. 81, Issue 4, pp. 505-12, (1995) (PubMed).

Details zu FADD

Target: FADD (Fas (TNFRSF6)-Associated Via Death Domain (FADD))

Andere Bezeichnung: FADD (FADD Produkte)

Synonyme: MORT1 antikoerper, Mort1/FADD antikoerper, Mort1 antikoerper, TNFRSF6 antikoerper, adaptor antikoerper, fadd antikoerper, mort1 antikoerper, xfadd antikoerper, Fas associated via death domain antikoerper, Fas (TNFRSF6)-associated via death domain antikoerper, Fas associated via death domain S homeolog antikoerper, Fas (tnfrsf6)-associated via death domain antikoerper, FADD antikoerper, Fadd antikoerper, fadd.S antikoerper, fadd antikoerper

Hintergrund: During apoptosis, cells exhibit morphological signs of the death process: cell shrinkage, membrane blebbing, and chromatin condensation. The role of the cell surface cytokine receptor, Fas (Apo-1, CD95), in apoptosis has been well characterized. The tumor necrosis factor receptor (TNF-R) can trigger cell death, as well as various other responses. Data suggested that Fas and TNF-R affect a common target in the cell death pathway. This target has been identified as FADD, a novel protein that contains a death domain homologous to the death domains of Fas and TNF-R1. FADD specifically binds to Fas, an association mediated by their homologous death domains. Overexpression of FADD induces apoptosis that is inhibited by CrmA, a poxvirus protein that blocks both Fas- and TNF-induced cell death. Thus, FADD is a central element of the Fas-mediated cell death pathway. This antibody is routinely tested by western blot analysis.

Molekulargewicht: 24 kDa

Pathways: Apoptose, TLR Signalweg, Activation of Innate immune Response, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades