NOS2 Antikörper (AA 961-1144)

Produktnummer ABIN967964

Produktdetails anti-NOS2 Antikörper

Target: NOS2 (Nitric Oxide Synthase 2, Inducible (NOS2))

Bindungsspezifität: AA 961-1144

Reaktivität: Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NOS2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse iNOS aa. 961-1144

Klon: 54-iNOS

Isotyp: IgG2a kappa

Anwendungsinformationen

Kommentare:

Related Products: ABIN968550, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Referenzen für anti-NOS2 Antikörper (ABIN967964)

Zadeh, Kolb, Geromin, DAnna, Boulmerka, Marconi, Dugas, Marsac, DAlessio: "Regulation of ICAM-1/CD54 expression on human endothelial cells by hydrogen peroxide involves inducible NO synthase." in: Journal of leukocyte biology, Vol. 67, Issue 3, pp. 327-34, (2000) (PubMed).

Resta, ODonaughy, Earley, Chicoine, Walker: "Unaltered vasoconstrictor responsiveness after iNOS inhibition in lungs from chronically hypoxic rats." in: The American journal of physiology, Vol. 276, Issue 1 Pt 1, pp. L122-30, (1999) (PubMed).

Zhao, Dugas, Mathiot, Delmer, Dugas, Sigaux, Kolb: "B-cell chronic lymphocytic leukemia cells express a functional inducible nitric oxide synthase displaying anti-apoptotic activity." in: Blood, Vol. 92, Issue 3, pp. 1031-43, (1998) (PubMed).

Vodovotz, Bogdan, Paik, Xie, Nathan: "Mechanisms of suppression of macrophage nitric oxide release by transforming growth factor beta." in: The Journal of experimental medicine, Vol. 178, Issue 2, pp. 605-13, (1993) (PubMed).

Xie, Cho, Calaycay, Mumford, Swiderek, Lee, Ding, Troso, Nathan: "Cloning and characterization of inducible nitric oxide synthase from mouse macrophages." in: Science (New York, N.Y.), Vol. 256, Issue 5054, pp. 225-8, (1992) (PubMed).

Details zu NOS2

Target: NOS2 (Nitric Oxide Synthase 2, Inducible (NOS2))

Andere Bezeichnung: iNOS/NOS Type II (NOS2 Produkte)

Synonyme: HEP-NOS antikoerper, INOS antikoerper, NOS antikoerper, NOS2A antikoerper, NOS-II antikoerper, Nos-2 antikoerper, Nos2a antikoerper, i-NOS antikoerper, iNOS antikoerper, iNos antikoerper, NOS2a antikoerper, NOS2 antikoerper, BmNOS2 antikoerper, Nsl antikoerper, nos2 antikoerper, nitric oxide synthase 2 antikoerper, nitric oxide synthase 2, inducible antikoerper, inducible nitric oxide synthase antikoerper, inducible nitric-oxide synthase antikoerper, nanos C2HC-type zinc finger 2 antikoerper, NOS2 antikoerper, Nos2 antikoerper, nos2 antikoerper, LOC396821 antikoerper, NANOS2 antikoerper

Hintergrund: Nitric oxide synthase (NOS), a cell-type specific enzyme, catalyzes the synthesis of nitric oxide (NO). NO is a short-lived radical that transmits cellular signals involved in vasorelaxation, neurotransmission, and cytotoxicity. In macrophages and other cell types, NOS (iNOS or macNOS) activity increases following exposure to cytokines (IFN-gamma, TNF-alpha, and IL-1) and microbial products (lipopolysaccharide (LPS)). iNOS is activated independently of Ca2+/calmodulin and its level of expression is tightly controlled by several transcription factors, including NFkappaB. Data indicates that TGF-beta affects translation of iNOS mRNA and decreases iNOS protein stability. Normally undetectable in brain tissue, iNOS mRNA has been observed in CNS tissues of animals under experimental pathologic conditions. iNOS and nNOS share 51% amino acid homology with the greatest degree of divergence in the calmodulin binding domain. This antibody has been reported to cross-react with nNOS and eNOS.
Synonyms: NOS Type II

Molekulargewicht: 130 kDa

Pathways: Retinoic Acid Receptor Signaling Pathway, Cellular Response to Molecule of Bacterial Origin, Inositol Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process