CDC27 Antikörper (AA 145-343)

Produktnummer ABIN967981

Produktdetails anti-CDC27 Antikörper

Target: CDC27 (Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27))

Bindungsspezifität: AA 145-343

Reaktivität: Human, Ratte, Maus, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CDC27 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Kreuzreaktivität: Hund, Ratte (Rattus), Maus

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human CDC27 aa. 145-343

Klon: 35-CDC27

Isotyp: IgG2b

Anwendungsinformationen

Kommentare:

Related Products: ABIN968535, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-CDC27 Antikörper (ABIN967981)

Saitoh, Pizzi, Wang: "Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4755-63, (2002) (PubMed).

Kramer, Scheuringer, Podtelejnikov, Mann, Peters: "Mitotic regulation of the APC activator proteins CDC20 and CDH1." in: Molecular biology of the cell, Vol. 11, Issue 5, pp. 1555-69, (2000) (PubMed).

Tugendreich, Tomkiel, Earnshaw, Hieter: "CDC27Hs colocalizes with CDC16Hs to the centrosome and mitotic spindle and is essential for the metaphase to anaphase transition." in: Cell, Vol. 81, Issue 2, pp. 261-8, (1995) (PubMed).

Tugendreich, Boguski, Seldin, Hieter: "Linking yeast genetics to mammalian genomes: identification and mapping of the human homolog of CDC27 via the expressed sequence tag (EST) data base." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 21, pp. 10031-5, (1993) (PubMed).

Details zu CDC27

Target: CDC27 (Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27))

Andere Bezeichnung: Cdc27 (CDC27 Produkte)

Synonyme: ANAPC3 antikoerper, APC3 antikoerper, CDC27Hs antikoerper, D0S1430E antikoerper, D17S978E antikoerper, HNUC antikoerper, NUC2 antikoerper, wu:fb54g06 antikoerper, wu:fc88c12 antikoerper, AI452358 antikoerper, BC023187 antikoerper, CDC27 antikoerper, NV10210 antikoerper, cell division cycle 27 antikoerper, cell division cycle 27 S homeolog antikoerper, cell division cycle protein 27 homolog antikoerper, anaphase-promoting complex subunit Apc3 antikoerper, CDC27 antikoerper, cdc27 antikoerper, Cdc27 antikoerper, cdc27.S antikoerper, LOC100123129 antikoerper, nuc2 antikoerper

Hintergrund: CDC16 and CDC27 were discovered in yeast as genes essential for the G2/M transition of the cell cycle. The human homologues of CDC16 and CDC27 were cloned and found to encode proteins of 72kDa and 97kDa, respectively. Biochemical and subcellular localization experiments have shown that CDC16 and CDC27 are novel components of the mitotic spindle centrosome in human cells. In HeLa cells, there are about 100,000 copies of each protein per cell. Although its precise role during mitosis remains to be determined, CDC16 appears to be required for cyclin degradation in yeast. Wild type CDC27 is required for the initiation of anaphase. Immunofluorescence studies have shown that both proteins are components of the centrosome in the absence of spindle fibers.

Molekulargewicht: 97 kDa