PP2A Catalytic alpha (AA 153-309) Antikörper

Produktnummer ABIN968034

Produktdetails

Target: PP2A Catalytic alpha

Bindungsspezifität: AA 153-309

Reaktivität: Hund, Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Applikation: Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)

Kreuzreaktivität: Hund, Ratte (Rattus), Maus

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human PP2A aa. 153-309

Klon: 46

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968533, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen

Efendiev, Yudowski, Zwiller, Leibiger, Katz, Berggren, Pedemonte, Leibiger, Bertorello: "Relevance of dopamine signals anchoring dynamin-2 to the plasma membrane during Na+,K+-ATPase endocytosis." in: The Journal of biological chemistry, Vol. 277, Issue 46, pp. 44108-14, (2002) (PubMed).

Nunbhakdi-Craig, Machleidt, Ogris, Bellotto, White, Sontag: "Protein phosphatase 2A associates with and regulates atypical PKC and the epithelial tight junction complex." in: The Journal of cell biology, Vol. 158, Issue 5, pp. 967-78, (2002) (PubMed).

Reiken, Gaburjakova, Guatimosim, Gomez, DArmiento, Burkhoff, Wang, Vassort, Lederer, Marks: "Protein kinase A phosphorylation of the cardiac calcium release channel (ryanodine receptor) in normal and failing hearts. Role of phosphatases and response to isoproterenol." in: The Journal of biological chemistry, Vol. 278, Issue 1, pp. 444-53, (2002) (PubMed).

Yang, Fan, Wadzinski, Sakurai, Richmond: "Protein phosphatase 2A interacts with and directly dephosphorylates RelA." in: The Journal of biological chemistry, Vol. 276, Issue 51, pp. 47828-33, (2001) (PubMed).

Chen, Parsons, Brautigan: "Tyrosine phosphorylation of protein phosphatase 2A in response to growth stimulation and v-src transformation of fibroblasts." in: The Journal of biological chemistry, Vol. 269, Issue 11, pp. 7957-62, (1994) (PubMed).

Antigendetails

Target: PP2A Catalytic alpha

Hintergrund: Protein phosphatases of the type 2A (PP2A) are composed of two regulatory and one catalytic subunit. The different subunits are encoded by multiple genes. PP2A are specific for phosphoserine and phosphothreonine residues of proteins that are involved in the regulation of cellular growth. PP2A binds and dephosphorylates the middle T antigens of polyoma virus. This interaction may regulate the transcriptional activity of the virus. The 36 kDa catalytic subunit is rapidly tyrosine phosphorylated as a result of growth factor stimulation or cell transformation. Tyrosine phosphorylation of the catalytic subunit may inactivate the phosphatase activity of PP2A.

Molekulargewicht: 36 kDa