PKA R1 alpha (PKR1) (AA 1-381) Antikörper

Produktnummer ABIN968061

Produktdetails

Target: PKA R1 alpha (PKR1)

Bindungsspezifität: AA 1-381

Reaktivität: Huhn, Hund, Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Applikation: Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)

Kreuzreaktivität: Ratte (Rattus), Hund, Maus, Huhn

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human PKA [RIalpha] aa. 1-381

Klon: 20-PKA RIalpha

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968545, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Referenzen

Taskén, Collas, Kemmner, Witczak, Conti, Taskén: "Phosphodiesterase 4D and protein kinase a type II constitute a signaling unit in the centrosomal area." in: The Journal of biological chemistry, Vol. 276, Issue 25, pp. 21999-2002, (2001) (PubMed).

Vang, Torgersen, Sundvold, Saxena, Levy, Skålhegg, Hansson, Mustelin, Taskén: "Activation of the COOH-terminal Src kinase (Csk) by cAMP-dependent protein kinase inhibits signaling through the T cell receptor." in: The Journal of experimental medicine, Vol. 193, Issue 4, pp. 497-507, (2001) (PubMed).

Casey, Vaughan, He, Hatcher, Winter, Weremowicz, Montgomery, Kucherlapati, Morton, Basson: "Mutations in the protein kinase A R1alpha regulatory subunit cause familial cardiac myxomas and Carney complex." in: The Journal of clinical investigation, Vol. 106, Issue 5, pp. R31-8, (2000) (PubMed).

Skålhegg, Landmark, Foss, Lohmann, Hansson, Lea, Jahnsen: "Identification, purification, and characterization of subunits of cAMP-dependent protein kinase in human testis. Reverse mobilities of human RII alpha and RII beta on sodium dodecyl sulfate-polyacrylamide gel electrophoresis compared with rat and bovine R" in: The Journal of biological chemistry, Vol. 267, Issue 8, pp. 5374-9, (1992) (PubMed).

Sandberg, Skålhegg, Jahnsen: "The two mRNA forms for the type I alpha regulatory subunit of cAMP-dependent protein kinase from human testis are due to the use of different polyadenylation site signals." in: Biochemical and biophysical research communications, Vol. 167, Issue 1, pp. 323-30, (1990) (PubMed).

Antigendetails

Target: PKA R1 alpha (PKR1)

Andere Bezeichnung: PKA RI alpha

Hintergrund: CAMP-dependent Protein Kinase (PKA) is composed of two distinct subunits: catalytic (C) and regulatory (R). Four regulatory subunits have been identified: RIalpha, RIbeta, RIIalpha, and RIIbeta. These subunits define type I and II cAMP-dependent protein kinases. Following binding of cAMP, the regulatory subunits dissociate from the catalytic subunits, rendering the enzyme active. Type I and type II holoenzymes have three potential C subunits (Calpha, Cbeta, or Cgamma). Type II PKA can be distinguished by autophosphorylation of the R-subunits, while type I PKA binds Mg/ATP with high affinity. Most cells express both type I and type II PKAs. Although the Ralpha isoforms are ubiquitously expressed, the Rbeta isoforms are predominant in nervous and adipose tissues. Expression of the RIbeta subunit is modulated during muscle and adipocyte differentiation in vitro.

Molekulargewicht: 49 kDa