ROCK2 Antikörper (AA 567-718)

Produktnummer ABIN968070

Produktdetails anti-ROCK2 Antikörper

Target: ROCK2 (rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2))

Bindungsspezifität: AA 567-718

Reaktivität: Human, Maus, Ratte, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ROCK2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Kreuzreaktivität: Maus, Hund, Human

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat ROKalpha aa. 567-718

Klon: 21-ROCK

Isotyp: IgG1

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-ROCK2 Antikörper (ABIN968070)

Begum, Sandu, Ito, Lohmann, Smolenski: "Active Rho kinase (ROK-alpha ) associates with insulin receptor substrate-1 and inhibits insulin signaling in vascular smooth muscle cells." in: The Journal of biological chemistry, Vol. 277, Issue 8, pp. 6214-22, (2002) (PubMed).

Wang, Eto, Steers, Somlyo, Somlyo: "RhoA-mediated Ca2+ sensitization in erectile function." in: The Journal of biological chemistry, Vol. 277, Issue 34, pp. 30614-21, (2002) (PubMed).

Adachi, Vita, Sannohe, Stafford, Alam, Kayaba, Chihara: "The functional role of rho and rho-associated coiled-coil forming protein kinase in eotaxin signaling of eosinophils." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 8, pp. 4609-15, (2001) (PubMed).

Sahai, Olson, Marshall: "Cross-talk between Ras and Rho signalling pathways in transformation favours proliferation and increased motility." in: The EMBO journal, Vol. 20, Issue 4, pp. 755-66, (2001) (PubMed).

Leung, Manser, Tan, Lim: "A novel serine/threonine kinase binding the Ras-related RhoA GTPase which translocates the kinase to peripheral membranes." in: The Journal of biological chemistry, Vol. 270, Issue 49, pp. 29051-4, (1996) (PubMed).

Details zu ROCK2

Target: ROCK2 (rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2))

Andere Bezeichnung: ROCK-II (ROCK2 Produkte)

Synonyme: rocka-a antikoerper, xROK antikoerper, rock2 antikoerper, rok2 antikoerper, ROCK2 antikoerper, B230113H15Rik antikoerper, ROKalpha antikoerper, Rho-kinase antikoerper, Rock-II antikoerper, Rock2m antikoerper, mKIAA0619 antikoerper, ROCK-II antikoerper, ROK antikoerper, Rho-associated, coiled-coil containing protein kinase 2 L homeolog antikoerper, rho-associated, coiled-coil containing protein kinase 2a antikoerper, Rho associated coiled-coil containing protein kinase 2 antikoerper, Rho-associated, coiled-coil containing protein kinase 2 antikoerper, Rho-associated coiled-coil containing protein kinase 2 antikoerper, rock2.L antikoerper, rock2a antikoerper, ROCK2 antikoerper, Rock2 antikoerper

Hintergrund: Activity of the GTP-binding proteins is regulated by GAPs, that accelerate binding, and GTPases, that enhance the rate of GTP hydrolysis (Ras, Rho, cdc42Hs, and Rac). The isoforms of Rho, a small GTP-binding protein, regulate cellular processes such as the formation of the stress fibers, lamelipodia, and filopodia. ROKalpha (RhoA-binding kinase) is a Ser/Thr protein kinase that interects with the GTP-binding form of RhoA. Like RhoA, ROKalpha localizes at the cellular margins and colocalizes with acti filaments. The ROKalpha gene encodes a protein of 1302 amino acids with homology to the human myotonic kinase. Although ROKalpha binds to GTP-RhoA, it is not yet clear whether this interaction induces the kinase activity of ROKalpha.
Synonyms: ROKalpha

Molekulargewicht: 180 kDa

Pathways: Microtubule Dynamics, WNT Signalweg, Tube Formation