SMAD2 Antikörper (AA 27-172)

Produktnummer ABIN968106

Produktdetails anti-SMAD2 Antikörper

Target: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Bindungsspezifität: AA 27-172

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SMAD2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Kreuzreaktivität: Maus, Ratte (Rattus)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human MAD2 aa. 27-172

Klon: 48-MAD2

Isotyp: IgG2a

Anwendungsinformationen

Kommentare:

Related Products: ABIN968537, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Referenzen für anti-SMAD2 Antikörper (ABIN968106)

Babu, Jeganathan, Baker, Wu, Kang-Decker, van Deursen: "Rae1 is an essential mitotic checkpoint regulator that cooperates with Bub3 to prevent chromosome missegregation." in: The Journal of cell biology, Vol. 160, Issue 3, pp. 341-53, (2003) (PubMed).

Iwanaga, Kasai, Kibler, Jeang: "Characterization of regions in hsMAD1 needed for binding hsMAD2. A polymorphic change in an hsMAD1 leucine zipper affects MAD1-MAD2 interaction and spindle checkpoint function." in: The Journal of biological chemistry, Vol. 277, Issue 34, pp. 31005-13, (2002) (PubMed).

Saitoh, Pizzi, Wang: "Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4755-63, (2002) (PubMed).

Chen, Waters, Salmon, Murray: "Association of spindle assembly checkpoint component XMAD2 with unattached kinetochores." in: Science (New York, N.Y.), Vol. 274, Issue 5285, pp. 242-6, (1996) (PubMed).

Li, Benezra: "Identification of a human mitotic checkpoint gene: hsMAD2." in: Science (New York, N.Y.), Vol. 274, Issue 5285, pp. 246-8, (1996) (PubMed).

Details zu SMAD2

Target: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Andere Bezeichnung: hsMAD2 (SMAD2 Produkte)

Synonyme: JV18 antikoerper, JV18-1 antikoerper, MADH2 antikoerper, MADR2 antikoerper, hMAD-2 antikoerper, hSMAD2 antikoerper, jv18 antikoerper, Xmad2 antikoerper, madh2 antikoerper, madr2 antikoerper, XSmad2 antikoerper, hmad-2 antikoerper, hsmad2 antikoerper, jv18-1 antikoerper, smad-2 antikoerper, fj43c06 antikoerper, wu:fj43c06 antikoerper, 7120426M23Rik antikoerper, Madh2 antikoerper, Madr2 antikoerper, Smad-2 antikoerper, mMad2 antikoerper, MAD homolog 2 antikoerper, Mothers against DPP homolog 2 antikoerper, SMAD 2 antikoerper, Smad2 antikoerper, SMAD family member 2 antikoerper, SMAD family member 2-Z antikoerper, SMAD family member 2 S homeolog antikoerper, SMAD2 antikoerper, SMAD2Z antikoerper, smad2 antikoerper, smad2.S antikoerper, Smad2 antikoerper

Hintergrund: Progression of the mammalian cell cycle is regulated by phosphorylation/dephosphorylation and synthesis/degradation of many key proteins. These events are of utmost importance at the checkpoints, or transition points, of the cell cycle. MAD2 (Mitotic Arrest Deficient) is the human homolog of a yeast and Xenopus protein that is essential for spindle assembly during mitosis. The human hsMAD2 gene encodes a protein of 205 amino acids with a predicted molecular weight of 23.5 kDa. Binding of affinity purified polyclonal antibodies to the MAD2 protein prevents mitosis of HeLa cells. This indicates that, like its invertebrate relatives, MAD2 is necessary for mitosis. Furthermore, MAD2 is localized at the kinetochore of condensed chromosomes during mitosis and cells defective in the mitotic checkpoint have reduced levels of MAD2.
Synonyms: Mitotic Arrest Deficient-2

Molekulargewicht: 24 kDa

Pathways: Zellzyklus, Hormone Transport, Chromatin Binding, Protein targeting to Nucleus