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MCM2 Antikörper (AA 725-888)

Dieses Anti-MCM2-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von MCM2 in WB, IHC, IF und IP. Geeignet für Human, Maus, Ratte, Hund und Huhn. Dieses Primary Antibody wurde in 3+ Publikationen zitiert.
Produktnummer ABIN968121

Kurzübersicht für MCM2 Antikörper (AA 725-888) (ABIN968121)

Target

Alle MCM2 Antikörper anzeigen
MCM2 (Minichromosome Maintenance Complex Component 2 (MCM2))

Reaktivität

  • 132
  • 73
  • 30
  • 10
  • 10
  • 9
  • 9
  • 5
  • 4
  • 4
  • 3
  • 2
  • 2
  • 1
Human, Maus, Ratte, Hund, Huhn

Wirt

  • 128
  • 15
  • 2
  • 1
Maus

Klonalität

  • 120
  • 26
Monoklonal

Konjugat

  • 88
  • 7
  • 6
  • 6
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
Dieser MCM2 Antikörper ist unkonjugiert

Applikation

  • 91
  • 58
  • 44
  • 32
  • 28
  • 26
  • 26
  • 26
  • 22
  • 17
  • 6
  • 4
  • 3
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Klon

46-BM28
  • Bindungsspezifität

    • 19
    • 16
    • 10
    • 8
    • 8
    • 7
    • 7
    • 6
    • 6
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 725-888

    Kreuzreaktivität

    Huhn, Hund, Maus, Ratte (Rattus)

    Produktmerkmale

    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

    Aufreinigung

    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

    Immunogen

    Human BM28 aa. 725-888

    Isotyp

    IgG1
  • Kommentare

    Related Products: ABIN968536, ABIN967389

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    250 μg/mL

    Buffer

    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    -20 °C

    Informationen zur Lagerung

    Store undiluted at -20° C.
  • Cook, Park, Burke, Leone, DeGregori, Engel, Nevins: "Analysis of Cdc6 function in the assembly of mammalian prereplication complexes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 3, pp. 1347-52, (2002) (PubMed).

    Shreeram, Sparks, Lane, Blow: "Cell type-specific responses of human cells to inhibition of replication licensing." in: Oncogene, Vol. 21, Issue 43, pp. 6624-32, (2002) (PubMed).

    Todorov, Pepperkok, Philipova, Kearsey, Ansorge, Werner: "A human nuclear protein with sequence homology to a family of early S phase proteins is required for entry into S phase and for cell division." in: Journal of cell science, Vol. 107 ( Pt 1), pp. 253-65, (1994) (PubMed).

  • Target

    MCM2 (Minichromosome Maintenance Complex Component 2 (MCM2))

    Andere Bezeichnung

    BM28

    Hintergrund

    BM28 is a phosphoprotein that migrates at 125kDa in SDS PAGE with a hyperphosphorylated form migrating as a slightly slower band. The ratio of the phosphorylation states alters with the phases of cell cycle - in M, the fast phosphorylated BM28 is the major form found, while in G1 phase, the slow, hyperphosphorylated BM28 predominates. The cellular localization of BM28 is also cell cycle dependent. In G1, most of the BM28 is chromatin bound, whereas, by M phase, the BM28 is still nuclear, but not associated with the DNA. BM28 is necessary for both entry into S phase and cell division as determined by microinjection inhibition experiments. Microinjection of a BM28 antibody into synchronised cells in G1 phase inhibits DNA replication. When injected during S phase or later, no effect on DNA replication is seen, but cell division is inhibited.

    Molekulargewicht

    125 kDa

    Pathways

    DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA
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