MCM2 Antikörper (AA 725-888)

Produktnummer ABIN968122

Produktdetails anti-MCM2 Antikörper

Target: MCM2 (Minichromosome Maintenance Complex Component 2 (MCM2))

Bindungsspezifität: AA 725-888

Reaktivität: Human, Maus, Ratte, Hund, Huhn

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MCM2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Kreuzreaktivität: Huhn, Hund, Maus, Ratte (Rattus)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human BM28 aa. 725-888

Klon: 46-BM28

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968536, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-MCM2 Antikörper (ABIN968122)

Cook, Park, Burke, Leone, DeGregori, Engel, Nevins: "Analysis of Cdc6 function in the assembly of mammalian prereplication complexes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 3, pp. 1347-52, (2002) (PubMed).

Shreeram, Sparks, Lane, Blow: "Cell type-specific responses of human cells to inhibition of replication licensing." in: Oncogene, Vol. 21, Issue 43, pp. 6624-32, (2002) (PubMed).

Ishimi, Ichinose, Omori, Sato, Kimura: "Binding of human minichromosome maintenance proteins with histone H3." in: The Journal of biological chemistry, Vol. 271, Issue 39, pp. 24115-22, (1996) (PubMed).

Todorov, Attaran, Kearsey: "BM28, a human member of the MCM2-3-5 family, is displaced from chromatin during DNA replication." in: The Journal of cell biology, Vol. 129, Issue 6, pp. 1433-45, (1995) (PubMed).

Todorov, Pepperkok, Philipova, Kearsey, Ansorge, Werner: "A human nuclear protein with sequence homology to a family of early S phase proteins is required for entry into S phase and for cell division." in: Journal of cell science, Vol. 107 ( Pt 1), pp. 253-65, (1994) (PubMed).

Details zu MCM2

Target: MCM2 (Minichromosome Maintenance Complex Component 2 (MCM2))

Andere Bezeichnung: BM28 (MCM2 Produkte)

Synonyme: CG7538 antikoerper, DmMCM2 antikoerper, DmMcm2 antikoerper, DmeMCM2 antikoerper, Dmel\\CG7538 antikoerper, MCM2 antikoerper, MCM2-7 antikoerper, MCM2_DROME antikoerper, McM2 antikoerper, PCR3 antikoerper, dMCM2 antikoerper, l(3)rL074 antikoerper, XMcm2 antikoerper, bm28 antikoerper, ccnl1 antikoerper, cdc19 antikoerper, cdcl1 antikoerper, mitotin antikoerper, cb737 antikoerper, chunp6905 antikoerper, BM28 antikoerper, CCNL1 antikoerper, CDCL1 antikoerper, D3S3194 antikoerper, MITOTIN antikoerper, MCM7 antikoerper, AA959861 antikoerper, AW476101 antikoerper, Mcmd2 antikoerper, mKIAA0030 antikoerper, Minichromosome maintenance 2 antikoerper, minichromosome maintenance complex component 2 antikoerper, minichromosome maintenance complex component 2 L homeolog antikoerper, Mcm2 antikoerper, mcm2 antikoerper, MCM2 antikoerper, mcm2.L antikoerper

Hintergrund: BM28 is a phosphoprotein that migrates at 125kDa in SDS PAGE with a hyperphosphorylated form migrating as a slightly slower band. The ratio of the phosphorylation states alters with the phases of cell cycle - in M, the fast phosphorylated BM28 is the major form found, while in G1 phase, the slow, hyperphosphorylated BM28 predominates. The cellular localization of BM28 is also cell cycle dependent. In G1, most of the BM28 is chromatin bound, whereas, by M phase, the BM28 is still nuclear, but not associated with the DNA. BM28 is necessary for both entry into S phase and cell division as determined by microinjection inhibition experiments. Microinjection of a BM28 antibody into synchronised cells in G1 phase inhibits DNA replication. When injected during S phase or later, no effect on DNA replication is seen, but cell division is inhibited.

Molekulargewicht: 125 kDa

Pathways: DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA