Flotillin 1 Antikörper (AA 312-428)

Produktnummer ABIN968194

Produktdetails anti-Flotillin 1 Antikörper

Target: Flotillin 1 (FLOT1)

Bindungsspezifität: AA 312-428

Reaktivität: Human, Maus, Ratte, Huhn

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Flotillin 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Kreuzreaktivität: Ratte (Rattus), Huhn, Human

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse Flotillin aa. 312-428

Klon: 18-Flotillin

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968545

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Referenzen für anti-Flotillin 1 Antikörper (ABIN968194)

Yamamori, Itakura, Sugaya, Katsumata, Sakagami, Takahashi: "Differential expression of SNAP-25 family proteins in the mouse brain." in: The Journal of comparative neurology, Vol. 519, Issue 5, pp. 916-32, (2011) (PubMed).

Cabin, Shimazu, Murphy, Cole, Gottschalk, McIlwain, Orrison, Chen, Ellis, Paylor, Lu, Nussbaum: "Synaptic vesicle depletion correlates with attenuated synaptic responses to prolonged repetitive stimulation in mice lacking alpha-synuclein." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 22, Issue 20, pp. 8797-807, (2002) (PubMed).

Fallon, Moreau, Croft, Labib, Gu, Fon: "Parkin and CASK/LIN-2 associate via a PDZ-mediated interaction and are co-localized in lipid rafts and postsynaptic densities in brain." in: The Journal of biological chemistry, Vol. 277, Issue 1, pp. 486-91, (2002) (PubMed).

Morrow, Rea, Martin, Prior, Prohaska, Hancock, James, Parton: "Flotillin-1/reggie-2 traffics to surface raft domains via a novel golgi-independent pathway. Identification of a novel membrane targeting domain and a role for palmitoylation." in: The Journal of biological chemistry, Vol. 277, Issue 50, pp. 48834-41, (2002) (PubMed).

Rybin, Xu, Lisanti, Steinberg: "Differential targeting of beta -adrenergic receptor subtypes and adenylyl cyclase to cardiomyocyte caveolae. A mechanism to functionally regulate the cAMP signaling pathway." in: The Journal of biological chemistry, Vol. 275, Issue 52, pp. 41447-57, (2001) (PubMed).

Details zu Flotillin 1

Target: Flotillin 1 (FLOT1)

Andere Bezeichnung: Flotillin-1 (FLOT1 Produkte)

Synonyme: reggie-2 antikoerper, flot1 antikoerper, Rareg antikoerper, flot1c antikoerper, flotillin-1 antikoerper, flotillin1 antikoerper, flot1a antikoerper, reggie2 antikoerper, si:dz44o19.1 antikoerper, wu:fj45g11 antikoerper, flotillin 1 antikoerper, flotillin-1 antikoerper, putative flotillin-1 antikoerper, flotillin 1 S homeolog antikoerper, flotillin 1 L homeolog antikoerper, flotillin 1a antikoerper, FLOT1 antikoerper, Flot1 antikoerper, flot1 antikoerper, LOC5566722 antikoerper, CpipJ_CPIJ008623 antikoerper, Smp_016200.1 antikoerper, flot1.S antikoerper, flot1.L antikoerper, flot1a antikoerper

Hintergrund: Caveolae are specialized membrane invaginations of 50-100 nm present in all cells, but abundant in endothelium, muscle cells, and adipocytes. These plasma membrane microdomains function in transcytosis of macromolecules, and are the sites of potocytosis, where small molecules are concentrated and transferred inside the cells by glycosylphosphatidylinositol (GPI)-linked receptors. Caveolin, a 22kDa protein and a well known marker for these plasma membrane microdomains, plays a structural role in these specializations. Flotillin-1 was isolated from the Triton X-100 insoluble buoyant fraction, characteristic of caveolae. Although the mRNA expression of both Flotillin-1 and Caveolin is very similar, Caveolin is undetectable in brain, while Flotillin-1 is very abundant. Flotillin-1 is a close homolog of the Epidermal Surface Antigen (ESA/Flotillin-2), which also colocalizes in the caveolae. Thus, Flotillin-1 and its relative ESA/Flotillin-2 are now incorporated into the expanding list of proteins co-localized at the caveolae which includes PKCalpha, Ras, Rap Src-like kinases, Galphaßgamma,and GPI-linked receptors. This antibody is routinely tested by western blot analysis.

Molekulargewicht: 48 kDa