Golgin A2 (GOLGA2) (AA 869-982) Antikörper

Produktnummer ABIN968196

Produktdetails

Target: Golgin A2 (GOLGA2)

Bindungsspezifität: AA 869-982

Reaktivität: Human, Maus, Ratte, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Kreuzreaktivität: Human, Hund, Maus

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat GM130 aa. 869-982

Klon: 35-GM130

Isotyp: IgG1 kappa

Anwendungsinformationen

Kommentare:

Related Products: ABIN968545, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen

Cox, Racca: "Differential dendritic targeting of AMPA receptor subunit mRNAs in adult rat hippocampal principal neurons and interneurons." in: The Journal of comparative neurology, Vol. 521, Issue 9, pp. 1954-2007, (2013) (PubMed).

Clarke, Emson, Irvine: "Distribution and neuronal expression of phosphatidylinositol phosphate kinase IIgamma in the mouse brain." in: The Journal of comparative neurology, Vol. 517, Issue 3, pp. 296-312, (2010) (PubMed).

Ireton, Davis, van Hengel, Mariner, Barnes, Thoreson, Anastasiadis, Matrisian, Bundy, Sealy, Gilbert, van Roy, Reynolds: "A novel role for p120 catenin in E-cadherin function." in: The Journal of cell biology, Vol. 159, Issue 3, pp. 465-76, (2002) (PubMed).

Marra, Maffucci, Daniele, Tullio, Ikehara, Chan, Luini, Beznoussenko, Mironov, De Matteis: "The GM130 and GRASP65 Golgi proteins cycle through and define a subdomain of the intermediate compartment." in: Nature cell biology, Vol. 3, Issue 12, pp. 1101-13, (2002) (PubMed).

Perez, Pernet-Gallay, Nizak, Goodson, Kreis, Goud: "CLIPR-59, a new trans-Golgi/TGN cytoplasmic linker protein belonging to the CLIP-170 family." in: The Journal of cell biology, Vol. 156, Issue 4, pp. 631-42, (2002) (PubMed).

Ralston, Lu, Ploug: "The organization of the Golgi complex and microtubules in skeletal muscle is fiber type-dependent." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 19, Issue 24, pp. 10694-705, (2000) (PubMed).

Nakamura, Lowe, Levine, Rabouille, Warren: "The vesicle docking protein p115 binds GM130, a cis-Golgi matrix protein, in a mitotically regulated manner." in: Cell, Vol. 89, Issue 3, pp. 445-55, (1997) (PubMed).

Antigendetails

Target: Golgin A2 (GOLGA2)

Andere Bezeichnung: GM130 (GOLGA2 Produkte)

Synonyme: gm130 antikoerper, MGC81213 antikoerper, AW555139 antikoerper, GM130 antikoerper, mKIAA4150 antikoerper, Gm130 antikoerper, golgin A2 S homeolog antikoerper, golgin A2 antikoerper, golgi autoantigen, golgin subfamily a, 2 antikoerper, golga2.S antikoerper, GOLGA2 antikoerper, Golga2 antikoerper

Hintergrund: Maturation and post-translational modification of proteins occurs after their biosynthesis at the endoplasmic reticulum and their transport through the Golgi apparatus. The process involves the transport of vesicles carrying the proteins through a vectorial process of vesicle budding and fusion from the cis-compartment to the medial-compartment and the trans-compartment of the Golgi apparatus. The detergent insoluble fraction of the Golgi is named matrix and is required for proper morphology of the Golgi membranes. GM130 (Golgi matrix protein of 130 kDa) is a protein isolated from the Triton™ X-100-insoluble Golgi matrix and peripherally associated with the cis-compartment, as demonstrated by co-localization with syntaxin5. GM130 is homologous to the Golgi autoantigen golgin 95. GM130 interacts through its N-terminal domain with p115 and with the Golgi membranes at the C-terminal portion. Furthermore, the mitotic phosphorylation of GM130 blocks the interaction with p115. Thus, GM130 appears to function as a structural element of the Golgi apparatus that also provides attachment sites for membranes and other Golgi proteins. The 35/GM130 monoclonal antibody recognizes GM130, regardless of phosphorylation status.

Molekulargewicht: 130 kDa

Pathways: SARS-CoV-2 Protein Interaktom